Rabbit Rel Polyclonal Antibody | anti-Rel antibody

Phospho-Rel (Ser492/Ser460) Antibody

Cat. Num. AAA9614127

• Gene Names: REL; C-Rel
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: Rel; Polyclonal Antibody; Phospho-Rel (Ser492/Ser460) Antibody; Avian reticuloendotheliosis; C REL; C Rel protein; c Rel proto oncogene protein; Oncogene REL; Oncogene REL avian reticuloendotheliosis; Proto-oncogene c-Rel; REL; REL_HUMAN; v rel avian reticuloendotheliosis viral oncogene homolog; v rel reticuloendotheliosis viral oncogene homolog; V rel reticuloendotheliosis viral oncogene homolog (avian); anti-Rel antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-Rel (Ser492/Ser460) Antibody detects endogenous levels of Rel only when phosphorylated at Ser492 (Isoform1,Q04864-1) or Ser460 (Isoform2,Q04864-2).
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-Rel antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:1000-3000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Rel around the phosphorylation site of Ser492/460.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Subunit Structure: Component of the NF-kappa-B p65-c-Rel complex. Component of the NF-kappa-B p50-c-Rel complex. Component of the NF-kappa-B p52-c-Rel complex. Homodimer; component of the NF-kappa-B c-Rel-c-Rel complex (By similarity). Interacts with NKIRAS1. Interacts with NFKBIB (By similarity). Interacts with NFKBIE.
• Subcellular Location: Nucleus.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from various samples, using Phospho-Rel (Ser492/Ser460) Antibody.Lane 1: Hela cells(serum starvation treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(serum starvation treatment),Lane 3: Rat brain,Lane 4: HepG2 cells(heat shock treatment),Lane 5: Mouse muscle.)

Western Blot (WB)

(Western blot analysis of extracts from Hepg2, using Rel (Phospho-Ser492/460) Antibody. Lane 1 was treated with the blocking peptide.)

Western Blot (WB)

(Western blot analysis of Rel (Phospho-Ser492/460) using Paclitaxel treated HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-Rel antibody

Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator.

NCBI and Uniprot Product Information

• NCBI GI #: 4506473
• NCBI GeneID: 5966
• NCBI Accession #: NP_002899.1
• NCBI GenBank Nucleotide #: NM_002908.2
• UniProt Accession #: Q04864; Q2PNZ7; Q6LDY0
• Molecular Weight: 68,520 Da
• NCBI Official Full Name: proto-oncogene c-Rel
• NCBI Official Synonym Full Names: v-rel reticuloendotheliosis viral oncogene homolog (avian)
• NCBI Official Symbol: REL
• NCBI Official Synonym Symbols: C-Rel
• NCBI Protein Information: proto-oncogene c-Rel; C-Rel proto-oncogene protein; oncogene REL, avian reticuloendotheliosis; v-rel avian reticuloendotheliosis viral oncogene homolog
• UniProt Protein Name: Proto-oncogene c-Rel
• Protein Family: Relaxin
• UniProt Gene Name: REL
• UniProt Entry Name: REL_HUMAN

NCBI Description

The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA (MIM 164014), RELB (604758), NFKB1 (MIM 164011), and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (MIM 164008) (Belguise and Sonenshein, 2007 [PubMed 18037997]).[supplied by OMIM, May 2008]

Uniprot Description

REL: a proto-oncogenic transcription factor of the nuclear factor-kappaB (NFkB) group. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. May play a role in differentiation and lymphopoiesis.

Protein type: Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 2p13-p12

Cellular Component: nucleoplasm; transcription factor complex; cytosol

Molecular Function: protein binding; chromatin binding; transcription factor activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 biosynthetic process; transcription, DNA-dependent; response to cytokine stimulus; cytokine production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; inflammatory response