Rabbit anti-Human RECQL5 Polyclonal Antibody | anti-RECQL5 antibody

RECQL5, ID (RECQL5, RECQ5, ATP-dependent DNA helicase Q5, DNA helicase, RecQ-like type 5, RecQ protein-like 5) (FITC)

Cat. Num. AAA6341194

• Gene Names: RECQL5; RECQ5
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RECQL5; Polyclonal Antibody; ID (RECQL5; RECQ5; ATP-dependent DNA helicase Q5; DNA helicase; RecQ-like type 5; RecQ protein-like 5) (FITC); RecQ protein-like 5; anti-RECQL5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RECQL5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RECQL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 662-691 amino acids from the Central region of human RECQL5.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RECQL5 Antibody (Center) western blot analysis in human Uterus tissue lysates (35ug/lane).This demonstrates the RECQL5 antibody detected the RECQL5 protein (arrow).)

Related Product Information for anti-RECQL5 antibody

RECQL5 may have an important role in DNA metabolism.

Product Categories/Family for anti-RECQL5 antibody

Antibodies; DNA Replication; Repair

References

Schwendener, S., et al. J. Biol. Chem. 285(21):15739-15745(2010). Islam, M.N., et al. Mol. Cell. Biol. 30(10):2460-2472(2010). Speina, E., et al. Nucleic Acids Res. 38(9):2904-2916(2010). Aygun, O., et al. J. Biol. Chem. 284(35):23197-23203(2009). Aygun, O., et al. Proc. Natl. Acad. Sci. U.S.A. 105(25):8580-8584(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 51242947
• NCBI GeneID: 9400
• NCBI Accession #: NP_001003715.1
• NCBI GenBank Nucleotide #: NM_001003715.3
• UniProt Accession #: O94762; Q9H0B1; Q9P1W7; Q9UNC8
• Molecular Weight: 108,858 Da
• NCBI Official Full Name: ATP-dependent DNA helicase Q5 isoform 2
• NCBI Official Synonym Full Names: RecQ protein-like 5
• NCBI Official Symbol: RECQL5
• NCBI Official Synonym Symbols: RECQ5
• NCBI Protein Information: ATP-dependent DNA helicase Q5; DNA helicase, RecQ-like type 5
• UniProt Protein Name: ATP-dependent DNA helicase Q5
• Protein Family: ATP-dependent DNA helicase
• UniProt Gene Name: RECQL5
• UniProt Synonym Gene Names: RECQ5; RecQ5
• UniProt Entry Name: RECQ5_HUMAN

Uniprot Description

RECQL5: May have an important role in DNA metabolism. Interacts with TOP3A and TOP3B. Ubiquitous. Belongs to the helicase family. RecQ subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleolus; DNA-directed RNA polymerase II, holoenzyme; nucleus

Molecular Function: DNA helicase activity; nucleic acid binding; ATP-dependent helicase activity; ATP binding

Biological Process: mitosis; cell division; DNA replication; DNA repair; DNA metabolic process; chromosome separation; DNA duplex unwinding; DNA recombination