Western Blot (WB)
(Western Blot (WB) analysis of RECQL4 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:Rat brain tissue lysate)
Rabbit RECQL4 Polyclonal Antibody | anti-RECQL4 antibody
RECQL4 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
RECQL4; Polyclonal Antibody; RECQL4 Polyclonal Antibody; ATP-dependent DNA helicase Q4; DNA helicase; RecQ-like type 4; RecQ4; RTS; RecQ protein-like 4; RECQ4; anti-RECQL4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RECQL4 polyclonal antibody detects endogenous levels of RECQL4 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
1208
Applicable Applications for anti-RECQL4 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human RECQL4.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of RECQL4 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:Rat brain tissue lysate)
Western Blot (WB)
(Western Blot (WB) analysis of RECQL4 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:Rat brain tissue lysate)
Related Product Information for anti-RECQL4 antibody
In humans, the RecQ helicase family includes WRN, BLM, RecQL1, RecQL4 and RecQL5 proteins, all of which contain a conserved helicase domain. WRN and BLM have been demonstrated to be the responsible genes in Werner and Bloom syndromes, respectively. RecQL1 and RecQL5 also belong to the human RecQ helicase family, but their correlation with genetic disorders, if any, is unknown. The gene encoding human RecQL4, which maps to chromosome 8q24.3, is believed to be the gene responsible for the development of Rothmund-Thomson syndrome (RTS). The levels of WRN, BLM, RecQL1, 4 and 5 are differentially up-regulated to guarantee genomic stability in cells that are transformed or actively proliferating. In humans, RecQL1 and RecQL5 map to chromosome 12p12 and 17q25, respectively.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
~ 133kDa
NCBI Official Full Name
ATP-dependent DNA helicase Q4
UniProt Protein Name
ATP-dependent DNA helicase Q4
Protein Family
UniProt Gene Name
RECQL4
UniProt Synonym Gene Names
RECQ4
UniProt Entry Name
RECQ4_HUMAN
Uniprot Description
RECQL4: DNA-dependent ATPase. May modulate chromosome segregation. Interacts with UBR1 and UBR2. Interacts with MCM10; this interaction regulates RECQL4 unwinding activity. Up-regulated in actively dividing cells. Ubiquitously expressed, with highest levels in thymus and testis. Belongs to the helicase family. RecQ subfamily.
Protein type: EC 3.6.4.12; Helicase
Chromosomal Location of Human Ortholog: 8q24.3
Cellular Component: membrane; cytoplasm; nucleus
Molecular Function: ATP-dependent 3'-5' DNA helicase activity; zinc ion binding; bubble DNA binding; ATP binding
Biological Process: multicellular organismal development; DNA strand renaturation; DNA repair; DNA replication; DNA duplex unwinding; DNA recombination
Disease: Rapadilino Syndrome; Rothmund-thomson Syndrome; Baller-gerold Syndrome
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Product Notes
The RECQL4 recql4 (Catalog #AAA3006391) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RECQL4 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RECQL4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the RECQL4 recql4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RECQL4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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