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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Rat Receptor Interacting Serine Threonine Kinase 3 (RIPK3) Polyclonal Antibody | anti-RIPK3 antibody

Polyclonal Antibody to Receptor Interacting Serine Threonine Kinase 3 (RIPK3)

Gene Names
Ripk3; Rip3; Hcyp2
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Receptor Interacting Serine Threonine Kinase 3 (RIPK3); Polyclonal Antibody; Polyclonal Antibody to Receptor Interacting Serine Threonine Kinase 3 (RIPK3); anti-RIPK3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against RIPK3. It has been selected for its ability to recognize RIPK3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- V KIVNSKKISR EVKAMVNLRH ENVLLLLGVT ENLEWDYVYG PALVTGFMEN GSLSGLLQPS CPRPWPLLCR LLEEVVLGMC YLHSLNPSLL HRDLKPSNVL LDLELHAKLA DFGLSTFQGG SQSGSGSGSR DSGGTLAYLA PELLDNDGKA SKASDVYSFG VLVWTVLAGR EAEVVDKTSL IRGAVCNRQR RPPLTELPPD SPETPGLEGL KELMTHCWSS EP
Sequence Length
518
Applicable Applications for anti-RIPK3 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant RIPK3 (Val50~Pro272) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2074882
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:RatTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:RatTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52,218 Da
NCBI Official Full Name
receptor-interacting serine/threonine-protein kinase 3
NCBI Official Synonym Full Names
receptor-interacting serine-threonine kinase 3
NCBI Official Symbol
Ripk3
NCBI Official Synonym Symbols
Rip3; Hcyp2
NCBI Protein Information
receptor-interacting serine/threonine-protein kinase 3
UniProt Protein Name
Receptor-interacting serine/threonine-protein kinase 3
UniProt Gene Name
Ripk3
UniProt Synonym Gene Names
Rip3; RIP-3

NCBI Description

a putative homocysteine respondent protein [RGD, Feb 2006]

Uniprot Description

RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; RIPK family; TKL group

Chromosomal Location of Human Ortholog: 15p13

Cellular Component: cytosol; intracellular; mitochondrion; plasma membrane

Molecular Function: ATP binding; identical protein binding; NF-kappaB-inducing kinase activity; protein complex binding; protein kinase activity; protein serine/threonine kinase activity; signal transducer activity

Biological Process: activation of protein kinase activity; I-kappaB kinase/NF-kappaB signaling; lymph node development; multicellular organism development; positive regulation of ligase activity; positive regulation of NF-kappaB transcription factor activity; positive regulation of oxidoreductase activity; protein autophosphorylation; protein heterooligomerization; protein homooligomerization; regulation of activated T cell proliferation; regulation of adaptive immune response; regulation of interferon-gamma production; regulation of T cell mediated cytotoxicity; spleen development; T cell differentiation in thymus; T cell homeostasis; thymus development

Research Articles on RIPK3

Similar Products

Product Notes

The RIPK3 ripk3 (Catalog #AAA2003562) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Receptor Interacting Serine Threonine Kinase 3 (RIPK3) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Receptor Interacting Serine Threonine Kinase 3 (RIPK3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the RIPK3 ripk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- V KIVNSKKISR EVKAMVNLRH ENVLLLLGVT ENLEWDYVYG PALVTGFMEN GSLSGLLQPS CPRPWPLLCR LLEEVVLGMC YLHSLNPSLL HRDLKPSNVL LDLELHAKLA DFGLSTFQGG SQSGSGSGSR DSGGTLAYLA PELLDNDGKA SKASDVYSFG VLVWTVLAGR EAEVVDKTSL IRGAVCNRQR RPPLTELPPD SPETPGLEGL KELMTHCWSS EP. It is sometimes possible for the material contained within the vial of "Receptor Interacting Serine Threonine Kinase 3 (RIPK3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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