Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RCC2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human RCC2 Polyclonal Antibody | anti-RCC2 antibody
RCC2 Polyclonal Antibody
Gene Names
RCC2; TD-60
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purification
Synonyms
RCC2; Polyclonal Antibody; RCC2 Polyclonal Antibody; TD-60; anti-RCC2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
QILPVPNVVVRDVACGANHTLVLDSQKRVFSWGFGGYGRLGHAEQKDEMVPRLVKLFDFPGRGASQIYAGYTCSFAVSEVGGLFFWGATNTSRESTMYPKAVQDLCGWRIRSLACGKSSIIVAADESTISWGPSPTFGELGYGDHKPKSSTAAQEVKTLDGIFSEQVAMGYSHSLVIARDESETEKEKIKKLPEYNPRTL
Sequence Length
522
Applicable Applications for anti-RCC2 antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human RCC2
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, nucleolus, spindle
Positive Samples
U-87MG, Jurkat
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RCC2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RCC2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Related Product Information for anti-RCC2 antibody
The protein encoded by this gene is a guanine exchange factor that is active on RalA, a small GTPase. The encoded protein and RalA are both essential for proper kinetochore-microtubule function in early mitosis. This protein has been shown to be a biomarker for colorectal cancer.
Product Categories/Family for anti-RCC2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 56kDa
Observed: 55kDa
Observed: 55kDa
NCBI Official Full Name
protein RCC2
NCBI Official Synonym Full Names
regulator of chromosome condensation 2
NCBI Official Symbol
RCC2
NCBI Official Synonym Symbols
TD-60
NCBI Protein Information
protein RCC2
UniProt Protein Name
Protein RCC2
Protein Family
UniProt Gene Name
RCC2
UniProt Synonym Gene Names
KIAA1470; TD60
NCBI Description
The protein encoded by this gene is a guanine exchange factor that is active on RalA, a small GTPase. The encoded protein and RalA are both essential for proper kinetochore-microtubule function in early mitosis. This protein has been shown to be a biomarker for colorectal cancer. [provided by RefSeq, Oct 2016]
Uniprot Description
Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.
Research Articles on RCC2
Similar Products
Product Notes
The RCC2 rcc2 (Catalog #AAA9133153) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RCC2 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RCC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the RCC2 rcc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QILPVPNVVV RDVACGANHT LVLDSQKRVF SWGFGGYGRL GHAEQKDEMV PRLVKLFDFP GRGASQIYAG YTCSFAVSEV GGLFFWGATN TSRESTMYPK AVQDLCGWRI RSLACGKSSI IVAADESTIS WGPSPTFGEL GYGDHKPKSS TAAQEVKTLD GIFSEQVAMG YSHSLVIARD ESETEKEKIK KLPEYNPRTL. It is sometimes possible for the material contained within the vial of "RCC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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