Rabbit KPNA3 Polyclonal Antibody | anti-KPNA3 antibody

KPNA3 Antibody

Cat. Num. AAA151270

• Gene Names: KPNA3; SRP1; SRP4; IPOA4; hSRP1; SRP1gamma
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: KPNA3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: KPNA3; Polyclonal Antibody; KPNA3 Antibody; SRP1; SRP4; IPOA4; hSRP1; SRP1gamma; QIP2; Importin subunit alpha-4; Importin alpha Q2; Qip2; karyopherin alpha 3 (importin alpha 4); anti-KPNA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: KPNA3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 521

• Applicable Applications for anti-KPNA3 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: KPNA3 antibody can be used for detection of KPNA3 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: KPNA3 antibody was raised against a 14 amino acid synthetic peptide near the carboxy end of human KPNA3.
• Buffer: KPNA3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: KPNA3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of KPNA3 in EL4 cell lysate with KPNA3 antibody at (A) 1 and (B) 2 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of KPNA3 in HeLa cells with KPNA3 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of KPNA3 in EL4 cells with KPNA3 antibody at 20 μg/mL.)

Related Product Information for anti-KPNA3 antibody

KPNA3 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA3, the alpha subunit 3 of karyopherin, is silimar to human KPNA2, suggesting that KPNA3 may be involved in the nuclear transport system. It is ubiquitously expressed and recent studies have shown that human KPNA3 may be associated with schizophrenia.

NCBI and Uniprot Product Information

• NCBI GI #: 34485722
• NCBI GeneID: 3839
• NCBI Accession #: NP_002258
• NCBI GenBank Nucleotide #: NM_002267.3
• UniProt Accession #: O00505; O00191; O43195; Q5JVM9; Q96AA7
• Molecular Weight: 57,811 Da
• NCBI Official Full Name: importin subunit alpha-4
• NCBI Official Synonym Full Names: karyopherin alpha 3 (importin alpha 4)
• NCBI Official Symbol: KPNA3
• NCBI Official Synonym Symbols: SRP1; SRP4; IPOA4; hSRP1; SRP1gamma
• NCBI Protein Information: importin subunit alpha-4; qip2; SRP1-gamma; importin alpha 4; importin alpha-3; importin alpha Q2; importin-alpha-Q2; importin subunit alpha-3; karyopherin subunit alpha-3
• UniProt Protein Name: Importin subunit alpha-4
• UniProt Gene Name: KPNA3
• UniProt Synonym Gene Names: QIP2; Qip2
• UniProt Entry Name: IMA4_HUMAN

NCBI Description

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. [provided by RefSeq, Jan 2009]

Uniprot Description

KPNA3: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9. Belongs to the importin alpha family.

Protein type: Adaptor/scaffold; Chaperone; Karyopherin; Nuclear import

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: nucleoplasm; nuclear pore; cytosol

Molecular Function: protein C-terminus binding; protein binding; nuclear localization sequence binding; protein transporter activity

Biological Process: entry of virus into host cell; cytokine and chemokine mediated signaling pathway; protein complex assembly; NLS-bearing substrate import into nucleus