Rabbit anti-Human RBMY1D Polyclonal Antibody | anti-RBMY1D antibody

RBMY1D, CT (RBMY1D, RNA-binding motif protein, Y chromosome, family 1 member D) (Biotin)

Cat. Num. AAA6340907

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RBMY1D; Polyclonal Antibody; CT (RBMY1D; RNA-binding motif protein; Y chromosome; family 1 member D) (Biotin); family 1 member D; anti-RBMY1D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RBMY1D antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RBMY1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-471 amino acids from the C-terminal region of human RBMY1D.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RBMY1D Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RBMY1D antibody detected the RBMY1D protein (arrow).)

Related Product Information for anti-RBMY1D antibody

This gene encodes a protein containing an RNA-binding motif in the N-terminus and four SRGY (serine, arginine, glycine, tyrosine) boxes in the C-terminus. Multiple copies of this gene are found in the AZFb azoospermia factor region of chromosome Y and the encoded protein is thought to be involved in spermatogenesis. Most copies of this locus are pseudogenes, although six highly similar copies have full-length ORFs and are considered functional. Four functional copies of this gene are found within inverted repeat IR2; two functional copies of this gene are found in palindrome P3, along with two copies of PTPN13-like, Y-linked. [provided by RefSeq].

Product Categories/Family for anti-RBMY1D antibody

Antibodies; Binding Proteins

References

Tsuei, D.J., et al. Oncogene 23(34):5815-5822(2004). Skaletsky, H., et al. Nature 423(6942):825-837(2003). Chai, N.N., et al. Genomics 49(2):283-289(1998). Prosser, J., et al. Mamm. Genome 7(11):835-842(1996). Ma, K., et al. Cell 75(7):1287-1295(1993).

NCBI and Uniprot Product Information

• NCBI GI #: 56090533
• NCBI GeneID: 378949
• NCBI Accession #: NP_001006120.2
• NCBI GenBank Nucleotide #: NM_001006120.2
• UniProt Accession #: P0C7P1
• Molecular Weight: 55,775 Da
• NCBI Official Full Name: RNA-binding motif protein, Y chromosome, family 1 member D
• NCBI Official Synonym Full Names: RNA binding motif protein, Y-linked, family 1, member D
• NCBI Official Symbol: RBMY1D
• NCBI Protein Information: RNA-binding motif protein, Y chromosome, family 1 member D
• UniProt Protein Name: RNA-binding motif protein, Y chromosome, family 1 member D
• Protein Family: RNA-binding motif protein
• UniProt Gene Name: RBMY1D
• UniProt Entry Name: RBY1D_HUMAN

NCBI Description

This gene encodes a protein containing an RNA-binding motif in the N-terminus and four SRGY (serine, arginine, glycine, tyrosine) boxes in the C-terminus. Multiple copies of this gene are found in the AZFb azoospermia factor region of chromosome Y and the encoded protein is thought to be involved in spermatogenesis. Most copies of this locus are pseudogenes, although six highly similar copies have full-length ORFs and are considered functional. Four functional copies of this gene are found within inverted repeat IR2; two functional copies of this gene are found in palindrome P3, along with two copies of PTPN13-like, Y-linked. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: RNA-binding protein which may be involved in spermatogenesis. Required for sperm development, possibly by participating in pre-mRNA splicing in the testis.

Subunit structure: Interacts with splicing factor proteins SFRS3/SRP20, TRA2B/SFRS10, KHDRBS1/SAM68 and KHDRBS3.

Subcellular location: Nucleus.

Tissue specificity: Testis-specific.

Developmental stage: Expressed in all of the transcriptionally active stages of germ cell development from spermatogonia through spermatocytes to round spermatids.

Miscellaneous: The RBMY1 proteins are encoded by repeated regions of the Y chromosome, mostly within the AZFb region. The exact number of functional copies is unclear and may vary between individuals, and some of them may represent pseudogenes. The proteins are very similar, which makes the characterization of each protein difficult. Thus, most experiments do not discriminate between the different members. One can therefore suppose that reported interactions with a RBMY1 protein involve all the proteins.

Sequence similarities: Contains 1 RRM (RNA recognition motif) domain.