Rabbit anti-Human RBM14 Polyclonal Antibody | anti-RBM14 antibody

RBM14, CT (RBM14, SIP, RNA-binding protein 14, Paraspeckle protein 2, RNA-binding motif protein 14, RRM-containing coactivator activator/modulator, Synaptotagmin-interacting protein) (Azide free) (HRP)

Cat. Num. AAA6340700

• Gene Names: RBM14; SIP; COAA; PSP2; SYTIP1; TMEM137
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RBM14; Polyclonal Antibody; CT (RBM14; SIP; RNA-binding protein 14; Paraspeckle protein 2; RNA-binding motif protein 14; RRM-containing coactivator activator/modulator; Synaptotagmin-interacting protein) (Azide free) (HRP); Synaptotagmin-interacting protein; anti-RBM14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 669

• Applicable Applications for anti-RBM14 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RBM14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 570-602 amino acids from the C-terminal region of human RBM14.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RBM14 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the RBM14 antibody detected the RBM14 protein (arrow).)

Immunohistochemistry (IHC)

(RBM14 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBM14 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RBM14 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RBM14 antibody

Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1.

Product Categories/Family for anti-RBM14 antibody

Antibodies; Syntaxin; Synaptotagmin; Synaptophysin

References

Li, X., et al. J. Cell. Biochem. 108(2):378-387(2009). Brooks, Y.S., et al. J. Biol. Chem. 284(27):18033-18046(2009). Kang, Y.K., et al. Cancer Res. 68(19):7887-7896(2008). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Matsuoka, S., et al. Science 316(5828):1160-1166(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 5454064
• NCBI GeneID: 10432
• NCBI Accession #: NP_006319.1
• NCBI GenBank Nucleotide #: NP_006319.1
• UniProt Accession #: Q96PK6
• NCBI Official Full Name: RNA-binding protein 14 isoform 1
• NCBI Official Synonym Full Names: RNA binding motif protein 14
• NCBI Official Symbol: RBM14
• NCBI Official Synonym Symbols: SIP; COAA; PSP2; SYTIP1; TMEM137
• NCBI Protein Information: RNA-binding protein 14
• UniProt Protein Name: RNA-binding protein 14
• Protein Family: RNA-binding protein
• UniProt Gene Name: RBM14
• UniProt Synonym Gene Names: SIP; PSP2; SYT-interacting protein
• UniProt Entry Name: RBM14_HUMAN

NCBI Description

This gene encodes a ribonucleoprotein that functions as a general nuclear coactivator, and an RNA splicing modulator. This protein contains two RNA recognition motifs (RRM) at the N-terminus, and multiple hexapeptide repeat domain at the C-terminus that interacts with thyroid hormone receptor-binding protein (TRBP), and is required for transcription activation. Alternatively spliced transcript variants encoding different isoforms (with opposing effects on transcription) have been described for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

RBM14: an RNA binding protein and transcriptional regulator. Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes. Two alternatively spliced isoforms have been observed. Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1.

Protein type: Nucleolus; RNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: nucleoplasm; transcription factor complex; nucleolus; Srb-mediator complex; ribonucleoprotein complex

Molecular Function: protein binding, bridging; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; RNA binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; estrogen receptor signaling pathway; glucocorticoid receptor signaling pathway; response to hormone stimulus; histone deacetylation; positive regulation of transcription from RNA polymerase II promoter; DNA repair; DNA replication; DNA recombination