Rabbit RBM14 Polyclonal Antibody | anti-RBM14 antibody

RBM14 Antibody

Cat. Num. AAA9612404

• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RBM14; Polyclonal Antibody; RBM14 Antibody; CoAA; Coactivator Activator; RBM 14; RNA binding motif protein 14; RNA binding protein 14; RRM containing coactivator activator/modulator; SIP; Synaptotagmin interacting protein; SYT interacting protein 1; SYT interacting protein; SYT interacting protein SIP; SYTIP 1; SYTIP1; anti-RBM14 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RBM14 Antibody detects endogenous levels of total RBM14.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 669

• Applicable Applications for anti-RBM14 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human RBM14, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, using RBM14 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-RBM14 antibody

Function: Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1 (PubMed:11443112). Regulates centriole biogenesis by suppressing the formation of aberrant centriolar protein complexes in the cytoplasm and thus preserving mitotic spindle integrity. Prevents the formation of the STIL-CENPJ complex (which can induce the formation of aberrant centriolar protein complexes) by interfering with the interaction of STIL with CENPJ (PubMed:25385835). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728).

Subcellular Location: Nucleus. Nucleus>Nucleolus. Cytoplasm. Note: In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles (PubMed:11790299). Cytoplasmic localization is crucial for its function in suppressing the formation of aberrant centriolar protein complexes (PubMed:25385835).

Tissue Specificity: Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes.

Subunit Structure: Isoform 1: Interacts with NCOA6, CITED1 and XRCC5/KU86 (PubMed:11443112). Isoform 1: Interacts with SS18 isoform 1 (PubMed:15919756). Isoform 1: Interacts with SS18 isoform 2 (PubMed:15919756). Interacts with STIL and interferes with its interaction with CENPJ. Interacts with gamma-tubulin (PubMed:25385835).Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA.

NCBI and Uniprot Product Information

• NCBI GI #: 73913750
• NCBI GeneID: 10052673710432
• UniProt Accession #: Q96PK6
• Molecular Weight: Observed Molecular Weight: (Observed)69kD.; Predicted Molecular Weight: (Calculated)69kDa.
• NCBI Official Full Name: RNA-binding protein 14
• UniProt Protein Name: RNA-binding protein 14
• Protein Family: RNA-binding protein
• UniProt Gene Name: RBM14
• UniProt Synonym Gene Names: SIP; PSP2; SYT-interacting protein
• UniProt Entry Name: RBM14_HUMAN

Uniprot Description

RBM14: an RNA binding protein and transcriptional regulator. Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes. Two alternatively spliced isoforms have been observed. Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1.

Protein type: Nucleolus; RNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: nucleoplasm; transcription factor complex; nucleolus; Srb-mediator complex; ribonucleoprotein complex

Molecular Function: protein binding, bridging; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; RNA binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; estrogen receptor signaling pathway; glucocorticoid receptor signaling pathway; response to hormone stimulus; histone deacetylation; positive regulation of transcription from RNA polymerase II promoter; DNA repair; DNA replication; DNA recombination