Western Blot (WB)
(Anti-RBKS rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:35 kDa)
Rabbit anti-Human RBKS Polyclonal Antibody | anti-RBKS antibody
Anti-RBKS Antibody, Rabbit Polyclonal
ELISA: 1:25,000-1:50,000
IHC-P: 1:12500-1:50,000
IP: 1-2uL/mg of lysate
Western Blot (WB)
(Anti-RBKS rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:35 kDa)
Western Blot (WB)
(Anti-RBKS rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:35 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human RBKS in human hepatoma with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human RBKS in human hepatoma with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)
Immunoprecipitation (IP)
(RBKS was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 1 uL anti-RBKS rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RBKS rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa Observed band size :35 kDa)
Immunoprecipitation (IP)
(RBKS was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 1 uL anti-RBKS rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RBKS rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa Observed band size :35 kDa)
NCBI and Uniprot Product Information
NCBI Description
The ribokinase encoded by this gene belongs to the pfkB family of carbohydrate kinases. It phosphorylates ribose to form ribose-5-phosphate in the presence of ATP and magnesium as a first step in ribose metabolism. [provided by RefSeq]
Uniprot Description
RBKS: The ribokinase encoded by this gene belongs to the pfkB family of carbohydrate kinases. It phosphorylates ribose to form ribose-5-phosphate in the presence of ATP and magnesium as a first step in ribose metabolism. [provided by RefSeq, Jul 2008]
Protein type: Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.15; Transferase
Chromosomal Location of Human Ortholog: 2p23.3
Molecular Function: ribokinase activity; ATP binding
Biological Process: carbohydrate phosphorylation; D-ribose catabolic process
Research Articles on RBKS
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Product Notes
The RBKS rbks (Catalog #AAA8107611) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RBKS Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RBKS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 IHC-P: 1:12500-1:50,000 IP: 1-2uL/mg of lysate. Researchers should empirically determine the suitability of the RBKS rbks for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBKS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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