Rabbit anti-Human RBCK1 Polyclonal Antibody | anti-RBCK1 antibody

RBCK1 Antibody, FITC conjugated

Cat. Num. AAA7053287

• Gene Names: RBCK1; XAP3; XAP4; HOIL1; PBMEI; PGBM1; RBCK2; RNF54; HOIL-1; ZRANB4; C20orf18; UBCE7IP3
• Reactivity: Human
• Applications: ELISA
• Purity: >95%, Protein G purified
• Synonyms: RBCK1; Polyclonal Antibody; RBCK1 Antibody; FITC conjugated; RanBP-type and C3HC4-type zinc finger-containing protein 1; HBV-associated factor 4; Heme-oxidized IRP2 ubiquitin ligase 1; HOIL-1; Hepatitis B virus X-associated protein 4; RING finger protein 54; Ubiquitin-conjugating enzyme 7-interacting protein 3; C20orf18; RNF54; UBCE7IP3; XAP3; XAP4; anti-RBCK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 468

• Applicable Applications for anti-RBCK1 antibody: ELISA (EIA)
• Immunogen: Recombinant Human RanBP-type and C3HC4-type zinc finger-containing protein 1 protein (162-261AA)
• Conjugation: FITC
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Related Product Information for anti-RBCK1 antibody

E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types.

NCBI and Uniprot Product Information

• NCBI GI #: 5454168
• NCBI GeneID: 10616
• NCBI Accession #: NP_006453.1
• NCBI GenBank Nucleotide #: NM_006462.5
• UniProt Accession #: Q9BYM8; O95623; Q86SL2; Q96BS3; Q9BYM9
• Molecular Weight: 25,654 Da
• NCBI Official Full Name: ranBP-type and C3HC4-type zinc finger-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: RANBP2-type and C3HC4-type zinc finger containing 1
• NCBI Official Symbol: RBCK1
• NCBI Official Synonym Symbols: XAP3; XAP4; HOIL1; PBMEI; PGBM1; RBCK2; RNF54; HOIL-1; ZRANB4; C20orf18; UBCE7IP3
• NCBI Protein Information: ranBP-type and C3HC4-type zinc finger-containing protein 1
• UniProt Protein Name: RanBP-type and C3HC4-type zinc finger-containing protein 1
• Protein Family: RanBP-type and C3HC4-type zinc finger-containing protein
• UniProt Gene Name: RBCK1
• UniProt Synonym Gene Names: C20orf18; RNF54; UBCE7IP3; XAP3; XAP4; HOIL-1

NCBI Description

The protein encoded by this gene is similar to mouse UIP28/UbcM4 interacting protein. Alternative splicing has been observed at this locus, resulting in distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

RBCK1: a E3 ubiquitin-protein ligase that contains a zf-RanBP (Zn-finger in Ran binding protein) and a zf-C3HC4 (RING finger) domain. Phosphorylation apparently reduces its E3 activity. Functions as a transcriptional activator. Its nuclear translocation is prevented by binding to a splice-variant of RBCK1 that lacks the RING finger domain. Interacts with PKCB1, PKCZ, and accepts ubiquitin from E2 ubiquitin-conjugating enzymes including UBE2L3. Interacts with PKCH. Interacts with the HBV pX/HBx protein, which is required to activate transcription of the viral genome. Three splice-variant isoforms of the human protein have been reported.

Protein type: EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: cytosol

Molecular Function: protein binding; ubiquitin binding; ubiquitin-protein ligase activity

Biological Process: activation of NF-kappaB transcription factor; I-kappaB kinase/NF-kappaB cascade; inhibition of NF-kappaB transcription factor; positive regulation of I-kappaB kinase/NF-kappaB cascade; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; T cell receptor signaling pathway

Disease: Polyglucosan Body Myopathy, Early-onset, With Or Without Immunodeficiency