Rabbit anti-Human RbAp48 Polyclonal Antibody | anti-RBBP4 antibody

Anti-RbAp48 Antibody, Rabbit Polyclonal

Cat. Num. AAA8105259

• Gene Names: RBBP4; NURF55; RBAP48; lin-53
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: RbAp48; Polyclonal Antibody; Anti-RbAp48 Antibody; Rabbit Polyclonal; RBBP4/RBAP48 Antibody; Rabbit PAb; Antigen Affinity Purified; retinoblastoma binding protein 4; Anti-lin-53 Antibody; Anti-NURF55 Antibody; Anti-RBAP48 Antibody; anti-RBBP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human RBBP4/RBAP48
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-RBBP4 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:2500-1:10,000; IP: 0.5-2uL/mg of lysate
• Immunogen: Recombinant Human RBBP4/RBAP48 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human RBBP4/RBAP48 (rh RBBP4/RBAP48). RBBP4/RBAP48 specific IgG was purified by Human RBBP4/RBAP48 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human RBBP4 in human testis with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human RBBP4 in human lung cancer with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human RBBP4 in human breast carcinoma with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Western Blot (WB)

(Anti-RBBP4 rabbit polyclonal antibody at 1:500 dilution.Lane A: Rat lung tissue lysate Lane B: Mouse spleen tissue lysate Lane C: Jurkat Whole Cell LysateLane D: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:48 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.))

Immunoprecipitation (IP)

(RBBP4 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-RBBP4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-RBBP4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 48 kDaObserved band size: 55 kDa)

Related Product Information for anti-RBBP4 antibody

Histone-binding protein RBBP4, also known as Retinoblastoma-binding protein 4, Retinoblastoma-binding protein p48, Chromatin assembly factor 1 subunit C, Chromatin assembly factor I p48 subunit, Nucleosome-remodeling factor subunit RBAP48 and RBBP4, is a nucleus protein which belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. RBBP4 is a component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling and the NURF (nucleosome remodeling factor) complex. One common myth is that age-related memory loss is an early indication of Alzheimer's disease. But researchers at the Columbia University Medical Center in New York City have found a specific protein, RbAp48, that they believe is responsible for age-related memory problems. What's more, by replenishing RbAp48 in the brains of mice, the researchers were able to undo existing age-related memory damage. To find RbAp48, researchers focused on the hippocampus, the region of the brain where memories are formed. After studying eight healthy brains donated to science by people between the ages of 33 and 88, they found that RbAp48 was reduced by nearly 5 percent in the older brains. The researchers found that when they turned off RbAp48 in younger mice, they became more forgetful, while increasing RbAp48 in older mice restored memory. The mice were given memory tests that included object recognition and water maze problems.

References

Rasmussen HH. et al., 1992, Electrophoresis 13:960-9. Qian Y.-W. et al., 1993, Nature 364:648-52. Yarden R.I. et al., 1999, Proc. Natl. Acad. Sci. USA. 96: 4983-8. Ota T. et al., 2004, Nat. Genet. 36:40-45. Gauci S. et al., 2009, Anal. Chem. 81:4493-501.

NCBI and Uniprot Product Information

• NCBI GI #: 5032027
• NCBI GeneID: 5928
• NCBI Accession #: NP_005601.1
• NCBI GenBank Nucleotide #: NM_005610.2
• UniProt Accession #: Q09028; P31149; Q53H02; Q96BV9; B2R6G9; B4DRH0; D3DPQ3
• Molecular Weight: 43,482 Da
• NCBI Official Full Name: histone-binding protein RBBP4 isoform a
• NCBI Official Synonym Full Names: retinoblastoma binding protein 4
• NCBI Official Symbol: RBBP4
• NCBI Official Synonym Symbols: NURF55; RBAP48; lin-53
• NCBI Protein Information: histone-binding protein RBBP4; RBBP-4; CAF-I p48; CAF-1 subunit C; CAF-I 48 kDa subunit; MSI1 protein homolog; retinoblastoma-binding protein 4; retinoblastoma-binding protein p48; chromatin assembly factor 1 subunit C; chromatin assembly factor I p48 sub
• UniProt Protein Name: Histone-binding protein RBBP4
• Protein Family: RbAp48-related WD40 repeat-containing protein
• UniProt Gene Name: RBBP4
• UniProt Synonym Gene Names: RBAP48; CAF-1 subunit C; CAF-I 48 kDa subunit; CAF-I p48; RBBP-4
• UniProt Entry Name: RBBP4_HUMAN

NCBI Description

This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

RBBP4: Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. Interacts with SUV39H1 and HDAC7. Binds directly to helix 1 of the histone fold of histone H4, a region that is not accessible when H4 is in chromatin. Subunit of the chromatin assembly factor 1 (CAF-1) complex, which is composed of RBBP4, CHAF1B and CHAF1A. Subunit of the core histone deacetylase (HDAC) complex, which is composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core HDAC complex associates with SIN3A, ARID4B/SAP180, SAP18, SAP30, SAP130, SDS3/SAP45 and possibly ARID4A/RBP1 and ING1 to form the SIN3 HDAC complex. The core HDAC complex may also associate with MTA2, MBD3, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylase complex (the NuRD complex). The NuRD complex may also interact with MBD3L1 and MBD3L2. Interacts with MTA1. Subunit of the PRC2/EED-EZH2 complex, which is composed of at least EED, EZH2, RBBP4, RBBP7 and SUZ12. The PRC2/EED-EZH2 complex may also associate with HDAC1. Component of the PRC2/EED- EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. Part of the nucleosome remodeling factor (NURF) complex which consists of SMARCA1; BPTF; RBBP4 and RBBP7. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SPEN/MINT. Interacts with BRCA1. Interacts with CREBBP, and this interaction may be enhanced by the binding of phosphorylated CREB1 to CREBBP. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Belongs to the WD repeat RBAP46/RBAP48/MSI1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: nucleoplasm; protein complex; Sin3 complex; ESC/E(Z) complex; nuclear chromatin; NuRD complex; nucleus

Molecular Function: DNA-dependent ATPase activity; protein binding; nucleosomal DNA binding; histone binding; histone deacetylase binding

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of cell cycle; chromatin assembly; regulation of gene expression, epigenetic; DNA replication-dependent nucleosome assembly; chromatin remodeling; negative regulation of cell proliferation; nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; DNA replication-independent nucleosome assembly; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; ATP-dependent chromatin remodeling; gene expression; mitotic cell cycle; G2/M transition of mitotic cell cycle; DNA replication