Rabbit anti-Human RB1 Polyclonal Antibody | anti-RB1 antibody

RB1 Antibody (S608)

Cat. Num. AAA9201020

• Gene Names: RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot, Immunofluorescence
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RB1; Polyclonal Antibody; RB1 Antibody (S608); Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110; anti-RB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-615 amino acids from human RB1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.35 (varies by lot)
• Sequence Positions: 586-615
• Sequence Length: 928

• Applicable Applications for anti-RB1 antibody: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Rb Antibody (S608) western blot analysis in Daudi,Raji cell line lysates (35ug/lane).This demonstrates the Rb antibody detected the Rb protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of RB1 Antibody (S608) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Immunohistochemistry (IHC)

(RB1 Antibody (S608) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RB1 Antibody (S608) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-RB1 antibody

The retinoblastoma protein is a tumor suppressor protein that is dysfunctional in many types of cancer.[1] One highly studied function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. pRb belongs to the pocket protein family, whose members have a pocket for the functional binding of other proteins.[2][3] Should an oncogenic protein, such as those produced by cells infected by high-risk types of human papillomaviruses, bind and inactivate pRb, this can lead to cancer. Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral.

Product Categories/Family for anti-RB1 antibody

Crown Antibodies; Cancer; Cell Biology

References

Dasgupta, P., et al., Mol. Cell. Biol. 24(21):9527-9541 (2004).
Cui, X., et al., Hum. Pathol. 35(10):1189-1195 (2004).
Borah, S., et al., J. Virol. 78(19):10336-10347 (2004).
Dasgupta, P., et al., J. Biol. Chem. 279(37):38762-38769 (2004).
Lohmann, D.R., et al., J. Biol. Chem. 129(1):23-28 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 108773787
• NCBI GeneID: 5925
• NCBI Accession #: NP_000312.2
• NCBI GenBank Nucleotide #: NM_000321.2
• UniProt Accession #: P06400; P78499; Q5VW46; Q8IZL4; A8K5E3
• Molecular Weight: 106159
• NCBI Official Full Name: retinoblastoma-associated protein
• NCBI Official Synonym Full Names: retinoblastoma 1
• NCBI Official Symbol: RB1
• NCBI Official Synonym Symbols: RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
• NCBI Protein Information: retinoblastoma-associated protein
• UniProt Protein Name: Retinoblastoma-associated protein
• Protein Family: RB1-inducible coiled-coil protein
• UniProt Gene Name: RB1
• UniProt Synonym Gene Names: Rb
• UniProt Entry Name: RB_HUMAN

NCBI Description

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Uniprot Description

Rb: retinoblastoma tumor suppressor protein regulates cell proliferation by controlling progression through the G1-phase restriction point. Has three distinct binding domains and interacts with regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by Cdks inhibits Rb target binding, thus allowing cell cycle progression. Recruits and targets histone methyltransferase suv39h1 leading to epigenetic transcriptional repression.

Protein type: Oncoprotein; Tumor suppressor; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 13q14.2

Cellular Component: nucleoplasm; SWI/SNF complex; Rb-E2F complex; PML body; spindle; chromatin; nucleus

Molecular Function: identical protein binding; protein binding; androgen receptor binding; DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; phosphoprotein binding; transcription factor activity; kinase binding; transcription factor binding

Biological Process: viral reproduction; sister chromatid biorientation; negative regulation of transcription from RNA polymerase II promoter, mitotic; positive regulation of transcription, DNA-dependent; regulation of mitotic cell cycle; neuron maturation; cell cycle arrest; positive regulation of macrophage differentiation; neurite development; negative regulation of epithelial cell proliferation; transcription, DNA-dependent; mitotic cell cycle checkpoint; negative regulation of transcription factor activity; regulation of lipid kinase activity; enucleate erythrocyte differentiation; G1/S-specific transcription in mitotic cell cycle; chromatin remodeling; myoblast differentiation; neuron apoptosis; maintenance of mitotic sister chromatid cohesion; gut development; cell division; neuron morphogenesis during differentiation; negative regulation of smoothened signaling pathway; Ras protein signal transduction; androgen receptor signaling pathway; positive regulation of mitotic metaphase/anaphase transition; striated muscle cell differentiation; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; cell cycle checkpoint; G1/S transition of mitotic cell cycle

Disease: Bladder Cancer; Osteogenic Sarcoma; Small Cell Cancer Of The Lung; Retinoblastoma