Rabbit anti-Human RASSF1 Polyclonal Antibody | anti-RASSF1 antibody

RASSF1 Antibody

Cat. Num. AAA9410073

• Gene Names: RASSF1; 123F2; RDA32; NORE2A; RASSF1A; REH3P21
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: RASSF1; Polyclonal Antibody; RASSF1 Antibody; 123F2; RDA32; NORE2A; RASSF1A; REH3P21; anti-RASSF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total RASSF1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 189

• Applicable Applications for anti-RASSF1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human RASSF1.
• Target Name: RASSF1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of 293T cell lysate using RASSF1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human stomach cancer using RASSF1 antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using RASSF1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-RASSF1 antibody

This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Product Categories/Family for anti-RASSF1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 332701155
• NCBI GeneID: 11186
• NCBI Accession #: NP_001193886.1
• NCBI GenBank Nucleotide #: NM_001206957.1
• UniProt Accession #: Q9NS23; O14571; O60539; O60710; Q0VGC6; Q5TZT2; Q9HB04; Q9HB18; Q9NS22; Q9UND4; Q9UND5; B7ZLL1
• Molecular Weight: 8,656 Da
• NCBI Official Full Name: ras association domain-containing protein 1 isoform B
• NCBI Official Synonym Full Names: Ras association (RalGDS/AF-6) domain family member 1
• NCBI Official Symbol: RASSF1
• NCBI Official Synonym Symbols: 123F2; RDA32; NORE2A; RASSF1A; REH3P21
• NCBI Protein Information: ras association domain-containing protein 1
• UniProt Protein Name: Ras association domain-containing protein 1
• Protein Family: Ras association domain-containing protein
• UniProt Gene Name: RASSF1
• UniProt Entry Name: RASF1_HUMAN

NCBI Description

This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, May 2011]

Uniprot Description

RASSF1: Potential tumor suppressor. Required for death receptor- dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage. Interacts with MAP1S and XPA. Binds to the N-terminal of CDC20 during prometaphase. Binds to STK3/MST2 and STK4/MST1. Isoform A interacts with MOAP1 and E4F1. Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization. Can self-associate. Isoform A interacts with RSSF5 and probably associates with HRAS1 via a RSSF1 isoform A-RSSF5 heterodimer. Part of a complex with MDM2, DAXX, RASSF1 and USP7. Isoform A interacts (via C-terminus) with DAXX (via N-terminus); the interaction is independent of MDM2 and TP53. Isoform C or isoform H interacts (via N-terminus) with DAXX. Isoform A interacts (via N-terminus) with MDM2 (via C-terminus); the interaction is independent of TP53. Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells. 8 isoforms of the human protein are produced by alternative promoter.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: microtubule cytoskeleton; spindle pole; microtubule; cytoplasm; plasma membrane; microtubule organizing center; nucleus

Molecular Function: identical protein binding; protein binding; zinc ion binding; protein N-terminus binding

Biological Process: protein stabilization; positive regulation of protein ubiquitination; Ras protein signal transduction; cell cycle arrest; response to DNA damage stimulus

Disease: Lung Cancer