IL-10 active protein

Human IL-10 Protein, His Tag

Cat. Num. AAA9718695

• Gene Names: IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
• Purity: >85% as determined by SDS-PAGE
• Synonyms: IL-10; Human IL-10 Protein; His Tag; Recombinant Human IL10 Protein; His Tag (Interleukin-10) expressed in Human Cells; CSIF; GVHDS; IL10A; Interleukin-10; TGIF; IL10; IL-10 active protein

• Host: Human Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence: Amino acid sequence derived from human IL10 (NP_000563.1) (Ser 19-Asn 178) was expressed, with a N-terminal polyhistidine tag.
• Sequence Length: 178

• Species: Human
• Endotoxin: The Endotoxin level is less than 1.0 EU per mug of the protein as determined by the LAL method
• Protein Length: The recombinant human IL10 comprises 176 amino acids and has a predicted molecular mass of 20.9 kDa as estimated in SDS-PAGE under reducing conditions.
• Activity: 1. Immobilized human IL10-His at 10 mug/ml (100 mul/well) can bind Cynomolgus IL10RA-Fc, The EC50 of Cynomolgus IL10RA-Fc is 0.31-0.71 mug/ml. 2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is typically 5-20 ng/mL.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL10 protein, His Tag in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human IL-10 protein, His Tag.)

Related Product Information for IL-10 active protein

IL-10 is a anti-inflammatory cytokine which belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production.

NCBI and Uniprot Product Information

• NCBI GI #: 10835141
• NCBI GeneID: 3586
• NCBI Accession #: NP_000563.1
• NCBI GenBank Nucleotide #: NP_000563.1
• UniProt Accession #: P22301
• Molecular Weight: 20.9 kDa
• NCBI Official Full Name: interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: IL10
• NCBI Official Synonym Symbols: CSIF; TGIF; GVHDS; IL-10; IL10A
• NCBI Protein Information: interleukin-10

NCBI Description

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]