Rabbit anti-Human Raptor Polyclonal Antibody | anti-RPTOR antibody

Raptor, Phosphorylated (Ser863) (Regulatory-associated Protein of mTOR, Raptor, P150 Target of Rapamycin (TOR)-scaffold Protein, RPTOR, KIAA1303) (AP)

Cat. Num. AAA6496920

• Gene Names: RPTOR; KOG1; Mip1
• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Raptor; Polyclonal Antibody; Phosphorylated (Ser863) (Regulatory-associated Protein of mTOR; P150 Target of Rapamycin (TOR)-scaffold Protein; RPTOR; KIAA1303) (AP); anti-RPTOR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Raptor when phosphorylated at Ser863.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 1335

• Applicable Applications for anti-RPTOR antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser863 of human Raptor (KLH).
• Conjugate: AP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-raptor-pS863 Pab (RB13350) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-RPTOR antibody

Antibodies; Protein Kinase Inhibitors

References

1. Wang, Y., Circ. Res. 101(6), 560-569 (2007). 2. Fuchs, B.C., Am. J. Physiol., Cell Physiol. 293(1), C55-C63 (2007). 3. Olsen, J.V., Cell 127(3), 635-648 (2006). 4. Megan Cully, et al. Mol. Cell. Biol., Jan 2010; 30: 481-495. (E-pub).

NCBI and Uniprot Product Information

• NCBI GI #: 22094987
• NCBI GeneID: 57521
• NCBI Accession #: NP_065812
• NCBI GenBank Nucleotide #: NP_065812.1
• UniProt Accession #: Q8N122
• Molecular Weight: 149.038
• NCBI Official Full Name: regulatory-associated protein of mTOR isoform 1
• NCBI Official Synonym Full Names: regulatory associated protein of MTOR complex 1
• NCBI Official Symbol: RPTOR
• NCBI Official Synonym Symbols: KOG1; Mip1
• NCBI Protein Information: regulatory-associated protein of mTOR
• UniProt Protein Name: Regulatory-associated protein of mTOR
• Protein Family: Regulatory-associated protein
• UniProt Gene Name: RPTOR
• UniProt Synonym Gene Names: KIAA1303; RAPTOR; Raptor

NCBI Description

This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis.