CREB Regulated Transcription Coactivator 3 Recombinant Protein | CRTC3 recombinant protein

Recombinant CREB Regulated Transcription Coactivator 3 (CRTC3)

Cat. Num. AAA2031302

• Gene Names: CRTC3; TORC3; TORC-3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: CREB Regulated Transcription Coactivator 3; Recombinant CREB Regulated Transcription Coactivator 3 (CRTC3); CRTC3 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MAASPGSGSA NPRKFSEKIA LHTQRQAEET RAFEQLMTDL TLSRVQFQKL QQLRLTQYHGGSLPNVSQLR SSASEFQPSF HQADNVRGTR HHGLVERPSR NRF
• Sequence Length: 619

• Applicable Applications for CRTC3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 15.4kDa
• Accurate Molecular Mass (KD): 15kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal tags, his-tag and T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Phe103
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 110431354
• NCBI GeneID: 64784
• NCBI Accession #: NP_073606.3
• NCBI GenBank Nucleotide #: NM_022769.4
• Molecular Weight: 66,830 Da
• NCBI Official Full Name: CREB-regulated transcription coactivator 3 isoform a
• NCBI Official Synonym Full Names: CREB regulated transcription coactivator 3
• NCBI Official Symbol: CRTC3
• NCBI Official Synonym Symbols: TORC3; TORC-3
• NCBI Protein Information: CREB-regulated transcription coactivator 3
• UniProt Protein Name: CREB-regulated transcription coactivator 3
• Protein Family: CREB-regulated transcription coactivator
• UniProt Gene Name: CRTC3
• UniProt Synonym Gene Names: TORC3; TORC-3; Transducer of CREB protein 3
• UniProt Entry Name: CRTC3_HUMAN

NCBI Description

This gene is a member of the CREB regulated transcription coactivator gene family. This family regulates CREB-dependent gene transcription in a phosphorylation-independent manner and may be selective for cAMP-responsive genes. The protein encoded by this gene may induce mitochondrial biogenesis and attenuate catecholamine signaling in adipose tissue. A translocation event between this gene and Notch coactivator mastermind-like gene 2, which results in a fusion protein, has been reported in mucoepidermoid carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

TORC3: Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). Binding, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1 enhances recruitment of TAF4 to the promoter. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interaction with HTLV-1 TAX enhances its transcriptional activity. Interacts, via the N- terminal with the ankyrin repeats of BCL3, to form a complex with CREB1 on CRE and TxRE responsive elements and represses HTLV-1 LTR-mediated transcription. Predominantly expressed in B and T lymphocytes. Highest levels in lung. Also expressed in brain, colon, heart, kidney, ovary, and prostate. Weak expression in liver, pancreas, muscle, small intestine, spleen and stomach. Belongs to the TORC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: cAMP response element binding protein binding

Biological Process: transcription, DNA-dependent; negative regulation of lipid catabolic process; viral reproduction; activation of CREB transcription factor; positive regulation of transcription from RNA polymerase II promoter; protein homotetramerization