Western Blot (WB)
(Western blot analysis of lysates from RAW264.7 cells, using RAPGEF5 Antibody. The lane on the right is blocked with the synthesized peptide.)
Rabbit RAPGEF5 Polyclonal Antibody | anti-RAPGEF5 antibody
RAPGEF5 Antibody
Gene Names
RAPGEF5; GFR; REPAC; MR-GEF
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
RAPGEF5; Polyclonal Antibody; RAPGEF5 Antibody; Guanine nucleotide exchange factor for Rap1; Related to Epac; Repac; M-Ras-regulated Rap GEF; MR-GEF; anti-RAPGEF5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
RAPGEF5 Antibody detects endogenous levels of total RAPGEF5 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
444
Applicable Applications for anti-RAPGEF5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:20000
ELISA: 1:20000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human RAPGEF5.
Immunogen Range
431-480
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Western Blot (WB)
(Western blot analysis of lysates from RAW264.7 cells, using RAPGEF5 Antibody. The lane on the right is blocked with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of lysates from RAW264.7 cells, using RAPGEF5 Antibody. The lane on the right is blocked with the synthesized peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67 kDa
NCBI Official Full Name
rap guanine nucleotide exchange factor 5
NCBI Official Synonym Full Names
Rap guanine nucleotide exchange factor 5
NCBI Official Symbol
RAPGEF5
NCBI Official Synonym Symbols
GFR; REPAC; MR-GEF
NCBI Protein Information
rap guanine nucleotide exchange factor 5
UniProt Protein Name
Rap guanine nucleotide exchange factor 5
Protein Family
UniProt Gene Name
RAPGEF5
UniProt Synonym Gene Names
GFR; KIAA0277; MRGEF; MR-GEF; Repac
NCBI Description
Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008]
Uniprot Description
Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation.
Research Articles on RAPGEF5
Similar Products
Product Notes
The RAPGEF5 rapgef5 (Catalog #AAA9506797) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAPGEF5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAPGEF5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:20000. Researchers should empirically determine the suitability of the RAPGEF5 rapgef5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAPGEF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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