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Western Blot (WB) (RAP2B Antibody (Center) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the RAP2B antibody detected the RAP2B protein (arrow).)

Rabbit anti-Human RAP2B Polyclonal Antibody

RAP2B, ID (RAP2B, Ras-related protein Rap-2b)

Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
RAP2B; Polyclonal Antibody; ID (RAP2B; Ras-related protein Rap-2b); Anti -RAP2B; anti-RAP2B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-RAP2B antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
RAP2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-129 amino acids from the Central region of human RAP2B.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RAP2B Antibody (Center) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the RAP2B antibody detected the RAP2B protein (arrow).)

Western Blot (WB) (RAP2B Antibody (Center) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the RAP2B antibody detected the RAP2B protein (arrow).)

Western Blot (WB)

(Western blot analysis of RAP2B (arrow) using rabbit polyclonal RAP2B Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAP2B gene.)

Western Blot (WB) (Western blot analysis of RAP2B (arrow) using rabbit polyclonal RAP2B Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAP2B gene.)
Product Categories/Family for anti-RAP2B antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI Official Full Name
Ras family small GTP binding protein RAP2B
Protein Family

Uniprot Description

RAP2B: Small GTP-binding protein which cycles between a GDP- bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells. Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein; G protein, monomeric, Ras; G protein, monomeric

Chromosomal Location of Human Ortholog: 3q25.2

Cellular Component: tight junction; membrane; recycling endosome membrane; plasma membrane; cytosol; lipid raft

Molecular Function: protein domain specific binding; GDP binding; GTP binding

Biological Process: microvillus biogenesis; platelet activation; signal transduction; positive regulation of protein amino acid autophosphorylation; negative regulation of cell migration; Rap protein signal transduction

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Product Notes

The RAP2B (Catalog #AAA642351) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAP2B, ID (RAP2B, Ras-related protein Rap-2b) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAP2B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the RAP2B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAP2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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