Rabbit RAP1A/RAP1B Polyclonal Antibody | anti-RAP1A antibody

RAP1A/RAP1B Rabbit pAb

Cat. Num. AAA4752214

• Gene Names: RAP1B; K-REV; RAL1B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: RAP1A/RAP1B; Polyclonal Antibody; RAP1A/RAP1B Rabbit pAb; Ras-related protein Rap-1b; RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21; anti-RAP1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-RAP1A antibody: Western Blot (WB), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 1:2000-1:10,000; FC/FACS: 1:20; IP: 1:20
• Immunogen: A synthetic peptide of human RAP1A/B
• Conjugation: Unconjugated
• Modification: Unmodification
• Subcellular Location: Cell junction, Cell membrane, Cytoplasm, Membrane
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of RAP1A/B in K562,Rat Brain,C6,3T3,Hela cell lysates using RAP1A/B Rabbit pAb(1:1000 diluted). Predicted band size:21kDa. Observed band size:21kDa.)

Related Product Information for anti-RAP1A antibody

This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function.

NCBI and Uniprot Product Information

• NCBI GI #: 47117723
• NCBI GeneID: 5908
• UniProt Accession #: P61224; P09526; Q502X3; Q5TZR4; Q6DCA1; Q6LES0; B2R5Z2; B4DQI8; B4DW74; B4DW94
• Molecular Weight: 184
• NCBI Official Full Name: Ras-related protein Rap-1b
• NCBI Official Synonym Full Names: RAP1B, member of RAS oncogene family
• NCBI Official Symbol: RAP1B
• NCBI Official Synonym Symbols: K-REV; RAL1B
• NCBI Protein Information: ras-related protein Rap-1b; RAS-related protein RAP1B; small GTP binding protein; GTP-binding protein smg p21B; Ras family small GTP binding protein RAP1B
• UniProt Protein Name: Ras-related protein Rap-1b
• Protein Family: Ras-related protein
• UniProt Gene Name: RAP1B
• UniProt Entry Name: RAP1B_HUMAN

NCBI Description

This gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 5, 6 and 9. [provided by RefSeq, Oct 2011]

Uniprot Description

RAP1B: GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Heterodimer with RAP1GAP. Interacts with EPAC2, SGSM1, SGSM2 and SGSM3. Activated by binding to the GTPase-activating protein RAP1GAP. Activated by guanine nucleotide-exchange factor (GEF) EPAC2 in a cAMP-dependent manner. Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein, monomeric, Ras; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: membrane; plasma membrane; lipid particle; intracellular; intercellular junction; cytosol; lipid raft

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; protein complex binding

Biological Process: platelet activation; cell proliferation; microvillus biogenesis; negative regulation of calcium ion-dependent exocytosis; metabolic process; blood coagulation; Rap protein signal transduction