Rabbit anti-Human RANK Polyclonal Antibody | anti-RANK antibody

RANK (Osteoclast differentiation factor receptor) (Biotin)

Cat. Num. AAA6404624

• Gene Names: TNFRSF11A; FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: RANK; Polyclonal Antibody; RANK (Osteoclast differentiation factor receptor) (Biotin); anti-RANK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RANK. No crossreactivity with other proteins.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.

• Applicable Applications for anti-RANK antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: WB: 2.5ng/lane under non-reducing and reducing conditions.; Applications are based on unconjugated antibody.
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human RANK.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot using 145118 Lane 1: Recombinant Human RANK Protein 10ng Lane 2: Recombinant Human RANK Protein 5ng Lane 3: Recombinant Human RANK Protein 2.5ng)

Immunohistochemistry (IHC)

(Immunohistochemistry in paraffin Human Mammary Cancer Tissue using 145118.)

Product Categories/Family for anti-RANK antibody

Antibodies; RANK

References

1. Suda T, Takahashi N, Udagawa N, Jimi E, Gillespie MT, Martin TJ (1999). "Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families". Endocr. Rev. 20 (3): 345-57. 2. Wong BR, Josien R, Choi Y (1999). "TRANCE is a TNF family member that regulates dendritic cell and osteoclast function". J. Leukoc. Biol. 65 (6): 715-24. 3. Theill LE, Boyle WJ, Penninger JM (2002). "RANK-L and RANK: T cells, bone loss, and mammalian evolution". Annu. Rev. Immunol. 20: 795-823.

NCBI and Uniprot Product Information

• NCBI GI #: 401015111
• NCBI GeneID: 8792
• NCBI Accession #: NP_001257878.1
• NCBI GenBank Nucleotide #: NM_001270949.1
• UniProt Accession #: Q9Y6Q6; Q59EP9; I4EC36; I4EC38; I4EC39; I7JE63; N0GVH0
• Molecular Weight: 64,552 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 11A isoform 2
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily member 11a
• NCBI Official Symbol: TNFRSF11A
• NCBI Official Synonym Symbols: FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 11A
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 11A
• UniProt Gene Name: TNFRSF11A
• UniProt Synonym Gene Names: RANK; ODFR
• UniProt Entry Name: TNR11_HUMAN

Uniprot Description

TNFRSF11A: Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. Binds to the clefts between the subunits of the TNFSF11 ligand trimer to form a heterohexamer. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Interacts (via cytoplasmic domain) with GAB2. Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q22.1

Cellular Component: external side of plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: cytokine binding; protein binding; receptor activity; transmembrane receptor activity; tumor necrosis factor receptor activity

Biological Process: activation of NF-kappaB transcription factor; adaptive immune response; cell-cell signaling; circadian thermoregulation; inflammatory response; osteoclast differentiation; positive regulation of cell proliferation; positive regulation of JNK activity; positive regulation of transcription factor activity; regulation of apoptosis; response to cytokine stimulus; response to lipopolysaccharide; signal transduction; tumor necrosis factor-mediated signaling pathway

Disease: Familial Expansile Osteolysis; Osteopetrosis, Autosomal Recessive 7; Paget Disease Of Bone