RAG2 Polyclonal Antibody | anti-RAG2 antibody

Anti-RAG2 Antibody

Cat. Num. AAA177723

• Gene Names: RAG2; RAG-2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: RAG2; Polyclonal Antibody; Anti-RAG2 Antibody; V(D)J recombination-activating protein 2; RAG 2; RAG-2; RAG2_HUMAN; Recombination activating gene 2; V(D)J recombination activating protein 2; recombination activating gene 2; anti-RAG2 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 527

• Applicable Applications for anti-RAG2 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RAG2 (498-527aa KKPPMKSLRKKGSGKILTPAKKSFLRRLFD), different from the related mouse sequence by three amino acids.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- RAG2 Picoband antibody, MBS177723, Western blottingAll lanes: Anti RAG2 (MBS177723) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: 22RV1 Whole Cell Lysate at 40ugLane 3: U20S Whole Cell Lysate at 40ugPredicted bind size: 59KDObserved bind size: 59KD )

Immunohistochemistry (IHC)

(Anti- RAG2 Picoband antibody, MBS177723, IHC(P)IHC(P): Mouse Spleen Tissue )

Immunohistochemistry (IHC)

(Anti- RAG2 Picoband antibody, MBS177723, IHC(P)IHC(P): Rat Spleen Tissue )

Immunohistochemistry (IHC)

(Anti- RAG2 Picoband antibody, MBS177723, IHC(P)IHC(P): Human Tonsil Tissue )

Related Product Information for anti-RAG2 antibody

Description: Rabbit IgG polyclonal antibody for V(D)J recombination-activating protein 2(RAG2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Recombination activating gene 2, also known as RAG-2, is a protein that in humans is encoded by the RAG2 gene. This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

References

1. "Entrez Gene: Recombination activating gene 2". 2. Callebaut, I., Mornon, J.-P. The V(D)J recombination activating protein RAG2 consists of a six-bladed propeller and a PHD fingerlike domain, as revealed by sequence analysis. Cell. Molec. Life Sci. 54: 880-891, 1998.

NCBI and Uniprot Product Information

• NCBI GI #: 151301080
• NCBI GeneID: 5897
• NCBI Accession #: NP_000527.2
• NCBI GenBank Nucleotide #: NM_000536.3
• UniProt Accession #: P55895; Q8TBL4; A8K9E9
• Molecular Weight: 59,241 Da
• NCBI Official Full Name: V(D)J recombination-activating protein 2
• NCBI Official Synonym Full Names: recombination activating gene 2
• NCBI Official Symbol: RAG2
• NCBI Official Synonym Symbols: RAG-2
• NCBI Protein Information: V(D)J recombination-activating protein 2
• UniProt Protein Name: V(D)J recombination-activating protein 2
• Protein Family: Seed allergenic protein
• UniProt Gene Name: RAG2
• UniProt Synonym Gene Names: RAG-2
• UniProt Entry Name: RAG2_HUMAN

NCBI Description

This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq, Jul 2008]

Uniprot Description

RAG2: Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T- lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B- cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Cells of the B- and T-lymphocyte lineages. Belongs to the RAG2 family.

Protein type: Enzyme, misc.

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: nucleoplasm

Molecular Function: chromatin binding; DNA binding; methylated histone residue binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol-4,5-bisphosphate binding; phosphoinositide binding; zinc ion binding

Biological Process: B cell differentiation; B cell homeostatic proliferation; B cell lineage commitment; chromatin modification; positive regulation of organ growth; pre-B cell allelic exclusion; protein ubiquitination; T cell differentiation in the thymus; T cell lineage commitment; V(D)J recombination

Disease: Combined Cellular And Humoral Immune Defects With Granulomas; Omenn Syndrome; Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-positive