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Western Blot (WB) (All lanes : Anti-RAE1 Antibody (N-Term) at 1:2000-1:4000 dilutionLane 1: Hela whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: MCF-7 whole cell lysateLane 5: SH-SY5Y whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human RAE1 Polyclonal Antibody | anti-RAE1 antibody

RAE1 Antibody (N-Term)

Gene Names
RAE1; MIG14; MRNP41; Mnrp41; dJ481F12.3; dJ800J21.1
Reactivity
Human
Applications
Western Blot
Synonyms
RAE1; Polyclonal Antibody; RAE1 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); mRNA export factor; Rae1 protein homolog; mRNA-associated protein mrnp 41; MRNP41; anti-RAE1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This RAE1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 19-42 amino acids from human RAE1.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
368
Applicable Applications for anti-RAE1 antibody
Western Blot (WB)
Application Notes
WB ~~ 1:2000-1:4000
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-RAE1 Antibody (N-Term) at 1:2000-1:4000 dilutionLane 1: Hela whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: MCF-7 whole cell lysateLane 5: SH-SY5Y whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-RAE1 Antibody (N-Term) at 1:2000-1:4000 dilutionLane 1: Hela whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: MCF-7 whole cell lysateLane 5: SH-SY5Y whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-RAE1 antibody
Binds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton.
Product Categories/Family for anti-RAE1 antibody
References
Bharathi A.,et al.Gene 198:251-258(1997). Kraemer D.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:9119-9124(1997). Lu L.,et al.Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004). Deloukas P.,et al.Nature 414:865-871(2001).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40968
NCBI Official Full Name
mRNA export factor
NCBI Official Synonym Full Names
ribonucleic acid export 1
NCBI Official Symbol
RAE1
NCBI Official Synonym Symbols
MIG14; MRNP41; Mnrp41; dJ481F12.3; dJ800J21.1
NCBI Protein Information
mRNA export factor
UniProt Protein Name
mRNA export factor
Protein Family
UniProt Gene Name
RAE1
UniProt Synonym Gene Names
MRNP41
UniProt Entry Name
RAE1L_HUMAN

NCBI Description

Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RAE1: Binds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Belongs to the WD repeat rae1 family.

Protein type: Motility/polarity/chemotaxis; RNA-binding

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: nuclear outer membrane; cytoskeleton; cytoplasm; nucleolus; nuclear pore; nuclear envelope; nucleus

Molecular Function: RNA binding; microtubule binding

Biological Process: viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; glucose transport; viral infectious cycle; post-translational protein modification; mRNA export from nucleus; protein sumoylation; cellular protein metabolic process; hexose transport; carbohydrate metabolic process; gene expression; viral transcription; mitotic cell cycle; transmembrane transport

Research Articles on RAE1

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Product Notes

The RAE1 rae1 (Catalog #AAA9215990) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAE1 Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:2000-1:4000. Researchers should empirically determine the suitability of the RAE1 rae1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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