Western Blot (WB)
((0.1ug/ml) staining of HepG2 (A), HeLa (B), Jurkat (C) and MCF7 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat RAE1 Polyclonal Antibody | anti-RAE1 antibody
Goat anti-RAE1 Antibody
Immunohistochemistry: In paraffin embedded Human Brain Cortex shows staining of neuronal cell bodies. Recommended concentration, 3-6ug/ml.
Western Blot: Approx 40kDa band observed in lysates cell lines HepG2, HeLa, Jurkat and MCF7 (calculated MW of 41. 0kDa according to NP_003601.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of HepG2 (A), HeLa (B), Jurkat (C) and MCF7 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of HepG2 (A), HeLa (B), Jurkat (C) and MCF7 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Brain Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Brain Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
RAE1: Binds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Belongs to the WD repeat rae1 family.
Protein type: Motility/polarity/chemotaxis; RNA-binding
Chromosomal Location of Human Ortholog: 20q13.31
Cellular Component: cytoplasm; nuclear envelope; nuclear pore; nucleolus; nucleus
Molecular Function: microtubule binding; RNA binding; ubiquitin binding
Biological Process: mitotic nuclear envelope disassembly; mRNA export from nucleus; protein import into nucleus; protein sumoylation; RNA-mediated gene silencing; tRNA export from nucleus; viral reproduction; viral transcription
Research Articles on RAE1
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Product Notes
The RAE1 rae1 (Catalog #AAA420468) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RAE1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's RAE1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Brain Cortex shows staining of neuronal cell bodies. Recommended concentration, 3-6ug/ml. Western Blot: Approx 40kDa band observed in lysates cell lines HepG2, HeLa, Jurkat and MCF7 (calculated MW of 41. 0kDa according to NP_003601.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the RAE1 rae1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NAAEELKPRN KK. It is sometimes possible for the material contained within the vial of "RAE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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