Typical Testing Data/Standard Curve (for reference only)
Monkey Calcium-binding mitochondrial carrier protein SCaMC-2 (SLC25A25) ELISA Kit | SLC25A25 elisa kit
Monkey Calcium-binding mitochondrial carrier protein SCaMC-2 (SLC25A25) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: SLC25A25 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SLC25A25 antibody and an SLC25A25-HRP conjugate. The assay sample and buffer are incubated together with SLC25A25-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC25A25 concentration since SLC25A25 from samples and SLC25A25-HRP conjugate compete for the anti-SLC25A25 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC25A25 from the sample, fewer sites are left to bind SLC25A25-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC25A25 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the family of calcium-binding mitochondrial carriers, with a characteristic mitochondrial carrier domain at the C-terminus. These proteins are found in the inner membranes of mitochondria, and function as transport proteins. They shuttle metabolites, nucleotides and cofactors through the mitochondrial membrane and thereby connect and/or regulate cytoplasm and matrix functions. This protein may function as an ATP-Mg/Pi carrier that mediates the transport of Mg-ATP in exchange for phosphate, and likely responsible for the net uptake or efflux of adenine nucleotides into or from the mitochondria. Alternatively spliced transcript variants encoding different isoforms with a common C-terminus but variable N-termini have been described for this gene. [provided by RefSeq, Jul 2012]
Uniprot Description
SLC25A25: Calcium-dependent mitochondrial solute carrier. Mitochondrial solute carriers shuttle metabolites, nucleotides, and cofactors through the mitochondrial inner membrane. May act as a ATP-Mg/Pi exchanger that mediates the transport of Mg-ATP in exchange for phosphate, catalyzing the net uptake or efflux of adenine nucleotides into or from the mitochondria. Belongs to the mitochondrial carrier family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter, SLC family; Mitochondrial; Membrane protein, integral; Membrane protein, multi-pass; Transporter
Chromosomal Location of Human Ortholog: 9q34.11
Cellular Component: intracellular membrane-bound organelle; mitochondrion; mitochondrial inner membrane; integral to membrane
Molecular Function: calcium ion binding
Biological Process: ATP metabolic process; response to food; response to dietary excess; camera-type eye development; multicellular organism growth; cellular respiration; response to activity
Research Articles on SLC25A25
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Product Notes
The Monkey SLC25A25 slc25a25 (Catalog #AAA7253609) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7253609 ELISA Kit recognizes Monkey SLC25A25. It is sometimes possible for the material contained within the vial of "Calcium-binding mitochondrial carrier protein SCaMC-2 (SLC25A25), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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