Goat RAD9A Polyclonal Antibody | anti-RAD9A antibody

Goat anti-RAD9A Antibody

Cat. Num. AAA420645

• Gene Names: RAD9A; RAD9
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Dog
• Applications: ELISA, Western Blot, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: RAD9A; Polyclonal Antibody; Goat anti-RAD9A Antibody; RAD9 homolog A (S. pombe); RAD9; RAD9 (S. pombe) homolog; RAD9 homolog; RAD9 homolog (S. pombe); cell cycle checkpoint control protein; RAD9A antibody; RAD9 homolog A (S. pombe) antibody; RAD9 antibody; RAD9 (S. pombe) homolog antibody; RAD9 homolog antibody; RAD9 homolog (S. pombe) antibody; cell cycle checkpoint control protein antibody; anti-RAD9A antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: QGPSPVLAEDSEGE
• Sequence Length: 391

• Applicable Applications for anti-RAD9A antibody: Peptide ELISA (EIA), Western Blot (WB), ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Approx 70kDa band observed in lysates of cell lines A431 and Daudi (calculated MW of 42.5kDa according to NP_004575.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Volkmer et al, J Biol Chem.1999 Jan 8;274(2): 567-70.; PMID: 9872989). Recommended concentration: 0.3-1ug/ml.; Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant RAD9A captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml.
• Immunogen: Peptide with sequence C-QGPSPVLAEDSEGE, from the C Terminus of the protein sequence according to NP_004575.1.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of A431cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Testing Data

((5ug/ml) as the reporter with as the capture rabbit antibody (5ug/ml).)

NCBI and Uniprot Product Information

• NCBI GI #: 4759022
• NCBI GeneID: 5883
• NCBI Accession #: NP_004575.1
• NCBI GenBank Nucleotide #: NM_004584.2
• Molecular Weight: 42,547 Da
• NCBI Official Full Name: cell cycle checkpoint control protein RAD9A isoform 1
• NCBI Official Synonym Full Names: RAD9 checkpoint clamp component A
• NCBI Official Symbol: RAD9A
• NCBI Official Synonym Symbols: RAD9
• NCBI Protein Information: cell cycle checkpoint control protein RAD9A
• UniProt Protein Name: Cell cycle checkpoint control protein RAD9A
• Protein Family: Cell cycle checkpoint control protein
• UniProt Gene Name: RAD9A
• UniProt Synonym Gene Names: hRAD9
• UniProt Entry Name: RAD9A_HUMAN

NCBI Description

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

RAD9A: a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. Possesses a 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. Forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for Rad9.

Protein type: Cell cycle regulation; DNA replication; EC 3.1.11.2; Apoptosis; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 11q13.1-q13.2

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: 3'-5' exonuclease activity; enzyme binding; exodeoxyribonuclease III activity; histone deacetylase binding; protein binding; protein kinase binding; SH3 domain binding

Biological Process: DNA damage checkpoint; DNA repair; DNA replication; DNA replication checkpoint; intra-S DNA damage checkpoint; response to DNA damage stimulus