Rabbit anti-Yeast RAD9, pan Polyclonal Antibody | anti-Rad9 antibody

RAD9, pan (aa1125-1139)

Cat. Num. AAA612805

• Gene Names: Rad9; CG3945; DmelCG3945; DmRad9; rad9
• Reactivity: Yeast
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: RAD9; pan; Polyclonal Antibody; pan (aa1125-1139); Anti -RAD9; anti-Rad9 antibody

• Host: Rabbit
• Reactivity: Yeast
• Clonality: Polyclonal
• Specificity: Data from both immunoblotting and ELISA indicate the antibody is pan reactive with both the phosphorylated and non-phosphorylated forms of the peptide and protein. Immunoblotting detects yeast Rad9 protein. No reactivity is expected against human and mouse homologs. Reactivity to Rad9 from others sources is unknown.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.

• Applicable Applications for anti-Rad9 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Immunoblot and ELISA.; Dilution: Immunoblot: 1:100-1:500; ELISA: 1:5000. 0.1ug of immunizing peptide was used in a standard capture ELISA using TMB as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher.
• Immunogen: Synthetic peptide corresponding to aa1125-1139 of 1309 of yeast Rad9 protein (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Rad9 antibody

Rad9 is required for the MEC1/TEL1dependent activation of Saccharomyces cerevisiae DNA damage checkpoint pathways mediated by Rad53 and Chk1. DNA damage induces Rad9 phosphorylation, and Rad53 specifically associates with phosphorylated Rad9. Cells have evolved multiple strategies for tolerating genomic damage. The most important of these are numerous repair systems that remove or bypass potentially mutagenic DNA lesions. Another cellular strategy is to delay cell-cycle transitions at multiple points. The genetic control of these delays, termed `checkpoints', was first established in budding yeast where it was shown that the RAD9 gene functions in G2/M arrest after irradiation with X-rays. Subsequently, it has become clear that Rad9 also functions at the G 1/S, intra-S and mid-anaphase checkpoints. Defects in checkpoint regulation can lead to genome instability and, in higher eukaryotes, neoplastic transformation. Rad9 also controls the transcriptional induction of a DNA damage regulon (DDR). Rad9 may also have a pro-apoptotic function. This is suggested in that Rad9 from Schizosaccharomyces pombe (SpRad9) contains a group of amino acids with similarity to the Bcl-2 homology 3 death domain, which is required for SpRad9 interaction with human Bcl-2 and apoptosis induction in human cells. Overexpression of Bcl-2 in S. pombe inhibits cell growth independently of rad9, but enhances resistance of rad9-null cells to methyl methanesulfonate, ultraviolet and ionizing radiation. Rad9 conveys the checkpoint signal by activating Rad53p and Chk1p; is hyperphosphorylated by Mec1p and Tel1p; and is a potential Cdc28p substrate. Mature yeast Rad9 is reported to have an apparent molecular weight of ~148kD. The human homolog is reported at 48.5kD.

Product Categories/Family for anti-Rad9 antibody

Antibodies; Abs to DNA Replication; Repair

NCBI and Uniprot Product Information

• NCBI GI #: 221512882
• NCBI GeneID: 40054
• NCBI Accession #: NP_001097639.2
• NCBI GenBank Nucleotide #: NM_001104169.3
• UniProt Accession #: O96533
• Molecular Weight: 50,988 Da
• NCBI Official Full Name: Rad9, isoform B
• NCBI Official Symbol: Rad9
• NCBI Official Synonym Symbols: CG3945; DmelCG3945; DmRad9; rad9
• NCBI Protein Information: CG3945 gene product from transcript CG3945-RA; CG3945-PA; CG3945-PB; Rad9; Rad9-PA; Rad9-PB
• UniProt Protein Name: DNA repair protein Rad9
• Protein Family: Protein
• UniProt Gene Name: Rad9
• UniProt Entry Name: O96533_DROME