Mouse Motility Related Protein (MRP1) ELISA Kit | MRP1 elisa kit

Mouse Motility Related Protein (MRP1) ELISA Kit

Cat. Num. AAA2122026

• Gene Names: CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
• Reactivity: Mouse
• Synonyms: Motility Related Protein (MRP1); Mouse Motility Related Protein (MRP1) ELISA Kit; CD9; TSPAN29; 5H9; BA2; BTCC-1; DRAP-27; GIG2; MIC3; P24; Tetraspanin 29; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; MRP1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Motility Related Protein (MRP1).; No significant cross-reactivity or interference between Motility Related Protein (MRP1) and analogues was observed.
• Sequence Length: 228

• Assay Type: Double-antibody Sandwich
• Sample: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Dectection Range: 31.2-2,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 11.9pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Motility Related Protein (MRP1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Motility Related Protein (MRP1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MRP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Motility Related Protein (MRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Motility Related Protein (MRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Motility Related Protein (MRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Motility Related Protein (MRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MRP1 elisa kit

Signal Transduction; Bone Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 34769
• NCBI GeneID: 928
• NCBI Official Full Name: MRP-1 (motility related protein)
• NCBI Official Synonym Full Names: CD9 molecule
• NCBI Official Symbol: CD9
• NCBI Official Synonym Symbols: MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
• NCBI Protein Information: CD9 antigen
• Protein Family: 37S ribosomal protein

NCBI Description

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]