Western Blot (WB)
(Western blot analysis of anti-Rad9 BH3 domain Pab in HL-60 cell lysate. Rad9 BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit anti-Human, Mouse Rad9 Polyclonal Antibody | anti-Rad9 antibody
Rad9, BH3 Domain (RAD9A, Cell Cycle Checkpoint Control Protein RAD9A, hRAD9, DNA Repair Exonuclease Rad9 Homolog A) (APC)
Gene Names
RAD9A; RAD9
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
Rad9; Polyclonal Antibody; BH3 Domain (RAD9A; Cell Cycle Checkpoint Control Protein RAD9A; hRAD9; DNA Repair Exonuclease Rad9 Homolog A) (APC); anti-Rad9 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Rad9. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-Rad9 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding within amino acid residues 1-100 of human Rad9 (KLH).
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of anti-Rad9 BH3 domain Pab in HL-60 cell lysate. Rad9 BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western blot analysis of anti-Rad9 BH3 domain Pab in HL-60 cell lysate. Rad9 BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western blot analysis of Rad9(arrow) using rabbit polyclonal Rad9 BH3 domain Pab. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Rad9 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of Rad9(arrow) using rabbit polyclonal Rad9 BH3 domain Pab. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Rad9 gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Product Categories/Family for anti-Rad9 antibody
References
1. Hopkins, K.M., et al., Cancer Res. 63(17):5291-5298 (2003). 2. Greer, D.A., et al., Cancer Res. 63(16):4829-4835 (2003). 3. St Onge, R.P., et al., J. Biol. Chem. 278(29):26620-26628 (2003). 4. Roos-Mattjus, P., et al., J. Biol. Chem. 278(27):24428-24437 (2003). 5. Yoshida, K., et al., EMBO J. 22(6):1431-1441 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,547 Da
NCBI Official Full Name
cell cycle checkpoint control protein RAD9A isoform 1
NCBI Official Synonym Full Names
RAD9 homolog A (S. pombe)
NCBI Official Symbol
RAD9A
NCBI Official Synonym Symbols
RAD9
NCBI Protein Information
cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD9
UniProt Protein Name
Cell cycle checkpoint control protein RAD9A
Protein Family
UniProt Gene Name
RAD9A
UniProt Synonym Gene Names
hRAD9
UniProt Entry Name
RAD9A_HUMAN
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Product Notes
The Rad9 rad9a (Catalog #AAA6496442) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rad9, BH3 Domain (RAD9A, Cell Cycle Checkpoint Control Protein RAD9A, hRAD9, DNA Repair Exonuclease Rad9 Homolog A) (APC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Rad9 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Rad9 rad9a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rad9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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