Rabbit Rad52 Polyclonal Antibody | anti-Rad52 antibody

Rad52 (Phospho-Y104) Polyclonal Antibody

Cat. Num. AAA3005428

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Rad52; Polyclonal Antibody; Rad52 (Phospho-Y104) Polyclonal Antibody; DNA repair protein RAD52 homolog; RAD52; anti-Rad52 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-Rad52 (Y104) polyclonal antibody detects endogenous levels of Rad52 protein when phosphorylated at Tyr104.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 418

• Applicable Applications for anti-Rad52 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human Rad52 around the phosphorylation site of Tyrosine 104.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-Rad52 (Y104) polyclonal antibody at 1:500 dilutionLane1:HepG2 cell lysateLane2:Mouse spleen tissue lysateLane3:Rat spleen tissue lysate)

Related Product Information for anti-Rad52 antibody

DNA double-strand breaks are generated by ionizing radiation and endogenously produced radicals, and they often are repaired through the RAD52 homologous recombination pathway. The RAD52 family includes RAD51, RAD52, RAD54, RAD54B and MRE11 genes. Rad51 and Rad52 proteins perform the key steps in homologous recombination (HR), including the search for DNA homology and strand exchange, through similar mechanisms. Mre11 functions in both non-homologous end joining, and meiotic HR, and it is essential in mitosis for chromosome maintenance. Rad54 belongs to the SWI2/SNF2 subfamily of ATPases, which includes the DNA helicases involved in replication, recombination, and repair, as it contains seven amino acid sequence motifs that are largely conserved.

NCBI and Uniprot Product Information

• NCBI GI #: 109637798
• NCBI GeneID: 5893
• NCBI Accession #: NP_602296.2
• NCBI GenBank Nucleotide #: NP_602296.2
• UniProt Accession #: P43351
• Molecular Weight: ~ 46kDa
• NCBI Official Full Name: DNA repair protein RAD52 homolog isoform a
• NCBI Official Synonym Full Names: RAD52 homolog, DNA repair protein
• NCBI Official Symbol: RAD52
• NCBI Protein Information: DNA repair protein RAD52 homolog
• UniProt Protein Name: DNA repair protein RAD52 homolog
• Protein Family: RAD52 motif-containing protein
• UniProt Gene Name: RAD52
• UniProt Entry Name: RAD52_HUMAN

NCBI Description

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]

Uniprot Description

RAD52: a protein involved in DNA double strand breaks and recombination. Associates with and is phosphorylated by c-Abl. Belongs to the RAD52/RAD22 family.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 12p13-p12.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding

Biological Process: DNA recombinase assembly; double-strand break repair; DNA repair; double-strand break repair via homologous recombination; DNA recombination