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Western Blot (WB) (Western blot analysis of extracts of K562 cell lines, using RAD52 antibody.)

Rabbit anti-Human RAD52 Polyclonal Antibody | anti-RAD52 antibody

RAD52 (DNA Repair Protein RAD52, RAD52 homolog (S. cerevisiae)) (APC)

Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
RAD52; Polyclonal Antibody; RAD52 (DNA Repair Protein RAD52; RAD52 homolog (S. cerevisiae)) (APC); anti-RAD52 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human RAD52.
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-RAD52 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
WB: 1:500-1:1000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to human RAD52.
Conjugate
APC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of K562 cell lines, using RAD52 antibody.)

Western Blot (WB) (Western blot analysis of extracts of K562 cell lines, using RAD52 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung tissue using RAD52 antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung tissue using RAD52 antibody.)
Product Categories/Family for anti-RAD52 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,169 Da
NCBI Official Full Name
DNA repair protein RAD52 homolog
NCBI Official Synonym Full Names
RAD52 homolog (S. cerevisiae)
NCBI Official Symbol
RAD52
NCBI Protein Information
DNA repair protein RAD52 homolog; recombination protein RAD52; rhabdomyosarcoma antigen MU-RMS-40.23
UniProt Protein Name
DNA repair protein RAD52 homolog
UniProt Gene Name
RAD52
UniProt Entry Name
RAD52_HUMAN

NCBI Description

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. [provided by RefSeq, Jul 2008]

Uniprot Description

RAD52: a protein involved in DNA double strand breaks and recombination. Associates with and is phosphorylated by c-Abl. Belongs to the RAD52/RAD22 family.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 12p13-p12.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding

Biological Process: DNA recombinase assembly; double-strand break repair; DNA repair; double-strand break repair via homologous recombination; DNA recombination

Research Articles on RAD52

Similar Products

Product Notes

The RAD52 rad52 (Catalog #AAA6434802) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAD52 (DNA Repair Protein RAD52, RAD52 homolog (S. cerevisiae)) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAD52 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:50-1:100 WB: 1:500-1:1000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RAD52 rad52 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAD52, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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