Rabbit anti-Human, Rat Rad23B/hHR23b Polyclonal Antibody | anti-Rad23B/hHR23b antibody

Rad23B/hHR23b (5H1) Rabbit pAb

Cat. Num. AAA4760305

• Gene Names: RAD23B; P58; HR23B; HHR23B
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: Rad23B/hHR23b; Polyclonal Antibody; Rad23B/hHR23b (5H1) Rabbit pAb; RAD23 homolog B; nucleotide excision repair protein; P58; HR23B; HHR23B; anti-Rad23B/hHR23b antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: 0.84mg/ml (varies by lot)

• Applicable Applications for anti-Rad23B/hHR23b antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:30-1:150
• Immunogen: Fusion protein of human RAD23B
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of Rad23B/hHR23b (5H1) in HT-29 cell, Rat brain tissue, 293T cell, HUVEC cell, Hela cell lysates in order using Rad23B/hHR23b (5H1) Rabbit pAb at dilution 1/350)

Immunohistochemistry (IHC)

(Immunohistochemistry of Rad23B/hHR23b (5H1) in paraffin-embedded Human lung cancer tissue using Rad23B/hHR23b (5H1) Rabbit pAb at dilution 1/25. Predicted cell location: Nucleus and Cytoplasm)

Related Product Information for anti-Rad23B/hHR23b antibody

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

NCBI and Uniprot Product Information

• NCBI GI #: 347800665
• NCBI GeneID: 5887
• NCBI Accession #: NP_001231653.1
• NCBI GenBank Nucleotide #: NM_001244724.1
• UniProt Accession #: P54727; Q7Z5K8; Q8WUB0; B3KWK8; G5E9P0
• Molecular Weight: 43,171 Da
• NCBI Official Full Name: UV excision repair protein RAD23 homolog B isoform 3
• NCBI Official Synonym Full Names: RAD23 homolog B (S. cerevisiae)
• NCBI Official Symbol: RAD23B
• NCBI Official Synonym Symbols: P58; HR23B; HHR23B
• NCBI Protein Information: UV excision repair protein RAD23 homolog B; RAD23, yeast homolog of, B; XP-C repair complementing protein; XP-C repair complementing complex 58 kDa; XP-C repair-complementing complex 58 kDa protein
• UniProt Protein Name: UV excision repair protein RAD23 homolog B
• UniProt Gene Name: RAD23B
• UniProt Synonym Gene Names: HR23B; hHR23B; p58
• UniProt Entry Name: RD23B_HUMAN

NCBI Description

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011]

Uniprot Description

RAD23B: Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum- associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. Belongs to the RAD23 family.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q31.2

Cellular Component: nucleoplasm; proteasome complex; cytoplasm; nucleus

Molecular Function: protein binding; damaged DNA binding; polyubiquitin binding; single-stranded DNA binding

Biological Process: nucleotide-excision repair, DNA damage recognition; proteasomal ubiquitin-dependent protein catabolic process; nucleotide-excision repair; regulation of proteasomal ubiquitin-dependent protein catabolic process; spermatogenesis; nucleotide-excision repair, DNA damage removal; DNA repair