Rabbit anti-Human RAD23A Polyclonal Antibody | anti-RAD23A antibody

RAD23A, CT (UV Excision Repair Protein RAD23 Homolog A, HHR23A)

Cat. Num. AAA626747

• Gene Names: RAD23A; MGC140027
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: RAD23A; Polyclonal Antibody; CT (UV Excision Repair Protein RAD23 Homolog A; HHR23A); Anti -RAD23A; anti-RAD23A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RAD23A.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-RAD23A antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human RAD23A (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RAD23A Antibody (C-term) in HL60 cell line lysates (35ug/lane). RAD23A (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-RAD23A antibody

RAD223A is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Product Categories/Family for anti-RAD23A antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 126717366
• NCBI GeneID: 540564
• UniProt Accession #: A3KMV2
• Molecular Weight: 39,589 Da
• NCBI Official Full Name: RAD23A protein
• NCBI Official Symbol: RAD23A
• NCBI Official Synonym Symbols: MGC140027
• NCBI Protein Information: UV excision repair protein RAD23 homolog A
• UniProt Protein Name: UV excision repair protein RAD23 homolog A
• Protein Family: UV excision repair protein
• UniProt Gene Name: RAD23A
• UniProt Entry Name: RD23A_BOVIN

Uniprot Description

Function: Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome

By similarity.Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, XPC:RAD23A dimer has NER activity. Can stabilize XPC

Subunit structure: Interacts with XPC; the interaction is suggesting the existence of a functional equivalent variant XPC complex. Interacts with PSMD4 and PSMC5. Interacts with ATXN3. Interacts with UBQLN2

Subcellular location: Nucleus

Probable.

Domain: The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit PSMD4 disrupt the UBL-UBA domain interactions and drive RAD23A in to an open conformation

Post-translational modification: Phosphorylated upon DNA damage, probably by ATM or ATR

Sequence similarities: Belongs to the RAD23 family.Contains 2 UBA domains.Contains 1 ubiquitin-like domain.