Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Cal27 Xenograft using RAD18 antibody at a dilution of 1:100)
Rabbit RAD18 Polyclonal Antibody | anti-RAD18 antibody
RAD18 Antibody
Gene Names
RAD18; RNF73
Applications
Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
RAD18; Polyclonal Antibody; RAD18 Antibody; Affinity Purified Rabbit Polyclonal RAD18 Antibody; Polyclonal RAD18 antibody; Anti-RAD18 antibody; RAD 18 antibody; RAD 18; RING finger protein 73 antibody; RNF73 antibody; postreplication repair protein hRAD18p antibody; E3 ubiquitin-protein ligase RAD18 antibody; RAD18 S. cerevisiae homolog antibody; postreplication repair protein RAD18 antibody; RAD-18; RAD18 antibody; RAD-18 antibody; hHR18 antibody; anti-RAD18 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.46 mg/ml (varies by lot)
Sequence Length
495
Applicable Applications for anti-RAD18 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 1:100-1:250
WB: 1:500-1:3000
WB: 1:500-1:3000
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Cal27 Xenograft using RAD18 antibody at a dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Cal27 Xenograft using RAD18 antibody at a dilution of 1:100)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 7.5% SDS PAGE gel and RAD18 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 7.5% SDS PAGE gel and RAD18 antibody at a dilution of 1:1000)
Related Product Information for anti-RAD18 antibody
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
Product Categories/Family for anti-RAD18 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
56 kDa
NCBI Official Full Name
RAD18
NCBI Official Synonym Full Names
RAD18 E3 ubiquitin protein ligase
NCBI Official Symbol
RAD18
NCBI Official Synonym Symbols
RNF73
NCBI Protein Information
E3 ubiquitin-protein ligase RAD18
Protein Family
NCBI Description
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]
Research Articles on RAD18
Similar Products
Product Notes
The RAD18 (Catalog #AAA5312445) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RAD18 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 1:100-1:250 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the RAD18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAD18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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