Rabbit anti-Human RACGAP1 Polyclonal Antibody | anti-RACGAP1 antibody

RACGAP1, NT (RACGAP1, KIAA1478, MGCRACGAP, Rac GTPase-activating protein 1, Male germ cell RacGap, Protein CYK4 homolg) (Azide free) (HRP)

Cat. Num. AAA6339936

• Gene Names: RACGAP1; CYK4; ID-GAP; HsCYK-4; MgcRacGAP
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RACGAP1; Polyclonal Antibody; NT (RACGAP1; KIAA1478; MGCRACGAP; Rac GTPase-activating protein 1; Male germ cell RacGap; Protein CYK4 homolg) (Azide free) (HRP); Protein CYK4 homolg; anti-RACGAP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RACGAP1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RACGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-58 amino acids from the N-terminal region of human RACGAP1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RACGAP1 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RACGAP1 antibody detected the RACGAP1 protein (arrow).)

Immunohistochemistry (IHC)

(RACGAP1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RACGAP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of RACGAP1 Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(RACGAP1 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-RACGAP1 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Seguin, L., et al. Mol. Cell. Biol. 29(2):570-581(2009). Simon, G.C., et al. EMBO J. 27(13):1791-1803(2008). Toure, A., et al. FEBS Lett. 582(8):1182-1188(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 186910300
• NCBI GeneID: 29127
• NCBI Accession #: NP_001119575.1; NP_037409.2
• NCBI GenBank Nucleotide #: NM_001126103.1
• UniProt Accession #: Q9H0H5; Q6PJ26; Q9NWN2; Q9P250; Q9P2W2
• Molecular Weight: 71,027 Da
• NCBI Official Full Name: rac GTPase-activating protein 1
• NCBI Official Synonym Full Names: Rac GTPase activating protein 1
• NCBI Official Symbol: RACGAP1
• NCBI Official Synonym Symbols: CYK4; ID-GAP; HsCYK-4; MgcRacGAP
• NCBI Protein Information: rac GTPase-activating protein 1; protein CYK4 homolg; protein CYK4 homolog; male germ cell RacGap; GTPase activating protein
• UniProt Protein Name: Rac GTPase-activating protein 1
• Protein Family: Rac GTPase-activating protein
• UniProt Gene Name: RACGAP1
• UniProt Synonym Gene Names: KIAA1478; MGCRACGAP; MgcRacGAP; CYK4; HsCYK-4
• UniProt Entry Name: RGAP1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the GTPase-activating protein (GAP) family. GAPs bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, GAPs negatively regulate Rho-mediated signals. This protein plays a regulatory role in initiation of cytokinesis, controlling cell growth and differentiation of hematopoietic cells, regulating spermatogenesis, and in neuronal proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

MgcRacGAP: a rac GTPase activating protein. Plays an important role in cytokinesis by regulating cortical movement through RhoA. Phosphorylation by Aurora B apparently induces GAP activity toward RhoA.

Protein type: GAPs; GAPs, Rac/Rho

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: nucleoplasm; microtubule; extrinsic to internal side of plasma membrane; acrosome; spindle midzone; midbody; cytosol; nucleus; cleavage furrow

Molecular Function: gamma-tubulin binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; microtubule binding; beta-tubulin binding; protein kinase binding; alpha-tubulin binding; GTPase activator activity

Biological Process: positive regulation of cytokinesis; regulation of attachment of spindle microtubules to kinetochore; spindle midzone assembly involved in mitosis; sulfate transport; antigen processing and presentation of exogenous peptide antigen via MHC class II; microtubule-based movement; regulation of small GTPase mediated signal transduction; embryonic development; cytokinesis after mitosis; small GTPase mediated signal transduction; neuroblast proliferation; spermatogenesis; blood coagulation; cytokinesis, contractile ring formation; positive regulation of GTPase activity