Rabbit anti-Human Rac1 Polyclonal Antibody | anti-Rac1 antibody

Anti-Rac1 Antibody

Cat. Num. AAA415718

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: Rac1; Polyclonal Antibody; Anti-Rac1 Antibody; anti-Rac1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 192

• Applicable Applications for anti-Rac1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA titer: 1:20,000-1:80,000; Western blot analysis: 1:1,000-1:2,000; Immunohistochemistry: 1:100-500; Optimal dilutions/concentrations should be determined by the end user
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Related Product Information for anti-Rac1 antibody

This gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

References

(1) Strausberg,R.L., Feingold,E.A., Grouse,L.H., et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)

NCBI and Uniprot Product Information

• NCBI GI #: 624236
• Molecular Weight: 23,467 Da
• NCBI Official Full Name: Rac1
• UniProt Protein Name: Ras-related C3 botulinum toxin substrate 1
• Protein Family: Ras-related protein
• UniProt Gene Name: RAC1
• UniProt Entry Name: RAC1_HUMAN

Uniprot Description

RAC1: a plasma membrane-associated member of the Rho-GTPase family. Plays a key role in cytoskeletal reorganization, membrane trafficking, transcriptional regulation and cell growth and development. GTP binding stimulates its activity. Phosphorylation by Akt may inhibit GTP binding of Rac1, therefore attenuating the downstream signal transduction pathway. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Two alternatively spliced isoforms have been described.

Protein type: G protein, monomeric; G protein, monomeric, Rho; G protein; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: Golgi membrane; extrinsic to plasma membrane; focal adhesion; membrane; lamellipodium; cytoplasm; plasma membrane; melanosome; actin filament; trans-Golgi network; cytosol; phagocytic cup

Molecular Function: GTPase activity; protein binding; enzyme binding; GTP binding; GTP-dependent protein binding; Rho GDP-dissociation inhibitor binding; thioesterase binding; protein kinase binding; Rab GTPase binding

Biological Process: axon guidance; nerve growth factor receptor signaling pathway; viral reproduction; metabolic process; positive regulation of apoptosis; cell-matrix adhesion; cell motility involved in cell locomotion; regulation of cell migration; localization within membrane; positive regulation of focal adhesion formation; actin filament polymerization; small GTPase mediated signal transduction; positive regulation of stress fiber formation; ephrin receptor signaling pathway; response to wounding; inflammatory response; cell adhesion; regulation of hydrogen peroxide metabolic process; bone resorption; lamellipodium biogenesis; intercellular junction assembly and maintenance; platelet activation; embryonic olfactory bulb interneuron precursor migration; anatomical structure morphogenesis; Wnt receptor signaling pathway, planar cell polarity pathway; mast cell chemotaxis; dendrite morphogenesis; positive regulation of Rho protein signal transduction; positive regulation of phosphoinositide 3-kinase activity; engulfment of apoptotic cell; regulation of defense response to virus by virus; cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of interleukin-23 production; hyperosmotic response; positive regulation of actin filament polymerization; organization of an anatomical structure; T cell costimulation; auditory receptor cell morphogenesis; ruffle organization and biogenesis; innate immune response; negative regulation of receptor-mediated endocytosis; cerebral cortex radially oriented cell migration; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; cell motility; blood coagulation; actin cytoskeleton organization and biogenesis; positive regulation of DNA replication