Rabbit Rabphilin 3A Polyclonal Antibody | anti-Rabphilin3A antibody

Rabphilin 3A Antibody

Cat. Num. AAA9613512

• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: Rabphilin 3A; Polyclonal Antibody; Rabphilin 3A Antibody; Exophilin 1; Exophilin-1; Exophilin1; KIAA0985; Rabphilin 3A homolog; Rabphilin-3A; Rabphilin3A; RP3A; RP3A_HUMAN; RpH3a; Rph3a; anti-Rabphilin3A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rabphilin 3A Antibody detects endogenous levels of total Rabphilin 3A.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-Rabphilin3A antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Rabphilin 3A, corresponding to a region within the internal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Ubiquitinated. Deubiquitinated by CAND1 to prevent its degradation.
• Subunit Structure: Interacts with RAB3B, RAB3C, RAB3D, RAB8A, RAB27A and RAB27B (By similarity). Interacts with RAB3A; this interaction recruits RPH3A to synaptic vesicules. Interacts (via C2B domain) with SNAP25 (By similarity). Interacts with deubiquitinating enzyme CAND1; this interaction results in the deubiquitination of RPH3A (By similarity). Interacts with GRIN2A and DLG4; this ternary complex regulates NMDA receptor composition at postsynaptic membranes (By similarity). Interacts with SNCA.
• Similarity: Binds calcium via the C2 domains. The calcium-bound C2 domains mediate interactions with phospholipid bilayers (By similarity).
• Subcellular Location: Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane. Cell projection>Dendritic spine. Cell junction>Synapse>Postsynaptic cell membrane. Membrane>Peripheral membrane protein.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from various samples, using Rabphilin 3A Antibody.Lane 1: 3T3, treated with blocking peptide;Lane 2: 3T3;Lane 3: Pc12.)

Western Blot (WB)

(Western blot analysis of Rabphilin 3A in lysates of HeLa TNF alpha 20ng/ml, 2mins, using Rabphilin 3A Antibody(AF4805).)

Related Product Information for anti-Rabphilin3A antibody

Plays an essential role in docking and fusion steps of regulated exocytosis (By similarity). At the presynaptic level, RPH3A is recruited by RAB3A to the synaptic vesicle membrane in a GTP-dependent manner where it modulates synaptic vesicle trafficking and calcium-triggered neurotransmitter release (By similarity). In the post-synaptic compartment, forms a ternary complex with GRIN2A and DLG4 and regulates NMDA receptor stability. Plays also a role in the exocytosis of arginine vasopressin hormone (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 219804203
• NCBI GeneID: 22895
• NCBI Accession #: NP_001137326.1
• NCBI GenBank Nucleotide #: NM_001143854.1
• UniProt Accession #: Q9Y2J0; Q96AE0; B7Z3C3
• Molecular Weight: 76,872 Da
• NCBI Official Full Name: rabphilin-3A isoform 1
• NCBI Official Synonym Full Names: rabphilin 3A homolog (mouse)
• NCBI Official Symbol: RPH3A
• NCBI Protein Information: rabphilin-3A; exophilin-1
• UniProt Protein Name: Rabphilin-3A
• UniProt Gene Name: RPH3A
• UniProt Synonym Gene Names: KIAA0985
• UniProt Entry Name: RP3A_HUMAN

NCBI Description

Exocytosis of neurotransmitters and hormones is fundamental to synaptic neurotransmission and cell-cell communication. RAB3A (MIM 179390) is a small G protein that is thought to act at late stages of exocytosis, and RPH3A is a RAB3A effector (Lin et al., 2007 [PubMed 17149709]).[supplied by OMIM, Jul 2008]

Uniprot Description

rabphilin 3A: Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: Golgi apparatus; synaptic vesicle; neuron projection; protein complex; extrinsic to membrane; synaptic vesicle membrane; synapse; cytosol; cell junction; secretory granule

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; selenium binding; protein binding; zinc ion binding; calcium-dependent phospholipid binding; transporter activity; protein complex binding; calcium ion binding; phosphate binding; Rab GTPase binding

Biological Process: intracellular protein transport