Rabbit anti Rat IgG1 (subclass specific) Secondary Antibody
Rabbit anti Rat IgG1 (subclass specific)
Applications
Immunodiffusion
Synonyms
Rabbit anti Rat IgG1 (subclass specific); Polyclonal Secondary Antibody; Rabbit anti Rat IgG1 (subclass specific) secondary antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Precipitating polyclonal Rabbit antiSerum to Rat IgG1, subclass specific.
Form/Format
Delipidated, heat inactivated, lyophilized whole antiserum. No preservative added, as it may interfere with the antibody activity Total protein and IgG concentration in the antiserum are comparable to those of pooled normal rabbit serum. No foreign protei
Applicable Applications for Rabbit anti Rat IgG1 (subclass specific) secondary antibody
Immunoelectrophoresis (IEP), Immunodiffusion (ID)
Application Notes
IEP: Use 2 ul or equivalent against 120 ul antiserum
ID: Use a rosette arrangement with 10 ul antiserum in a 3 mm diameter centre well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells.
ID: Use a rosette arrangement with 10 ul antiserum in a 3 mm diameter centre well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivity Note
This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail, however in double radial immunodiffusion a reaction with Mouse and a weak reaction with Human has been observed.
Preparation and Storage
Store at 4°C. Avoid repeated thawing and freezing.
Related Product Information for Rabbit anti Rat IgG1 (subclass specific) secondary antibody
The reactivity of the antiserum is directed to the subclass IgG1. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG1 in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgG1 from serum or plasma.
Product Categories/Family for Rabbit anti Rat IgG1 (subclass specific) secondary antibody
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Product Notes
The Rabbit anti Rat IgG1 (subclass specific) (Catalog #AAA571654) is a Secondary Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Rabbit anti Rat IgG1 (subclass specific) can be used in a range of immunoassay formats including, but not limited to, Immunoelectrophoresis (IEP), Immunodiffusion (ID). IEP: Use 2 ul or equivalent against 120 ul antiserum ID: Use a rosette arrangement with 10 ul antiserum in a 3 mm diameter centre well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells. Researchers should empirically determine the suitability of the Rabbit anti Rat IgG1 (subclass specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rabbit anti Rat IgG1 (subclass specific), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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