Rabbit anti Human Ig kappa light chain (free) Secondary Antibody
Rabbit anti Human Ig kappa light chain (free)
Reactivity
Human
Applications
Precipitation assays.
Synonyms
Rabbit anti Human Ig kappa light chain (free); Polyclonal Secondary Antibody; Rabbit anti Human Ig kappa light chain (free) secondary antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Precipitating polyclonal Rabbit antiSerum to free Human Ig kappa chain.
Form/Format
Delipidated, heat inactivated lyophilized whole serum. No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles
Applicable Applications for Rabbit anti Human Ig kappa light chain (free) secondary antibody
Precipitation assays.
Host Note
A pool of purified Bence Jones kappa proteins isolated from human urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Reactivity Note
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.
Preparation and Storage
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Related Product Information for Rabbit anti Human Ig kappa light chain (free) secondary antibody
The reactivity of the antiserum is directed to the hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is only reacting with Bence Jones proteins as well as free light chains of the kappa type. No reaction is obtained with intact polyclonal or monoclonal immunoglobulins. To detect and identify free light chain type and Bence Jones protein in serum or urine by To detect and identify free light chain type and Bence Jones protein in serum or urine by immunoelectrophoresis or double radial immunodiffusion. It is of great importance as an aid in the identification of B cell malignancies such a multiple myeloma and non-Hodgkin lymphoma, and in monitoring their therapy. In immunoelectrophoresis use 1.2 ?l of sample in a well with a diameter of 1 mm against 60 ?l antiserum in a 20 mm long channel. Electrophoresis for 1 hour, 20 mA. The detection limit of this technique in serum is about 25 mg/l; in the case 100 times concentrated urine is used the detection limit can be brought down to 0.5 mg/l. The fact that in about 40% of the case the Bence Jones protein was only detectable in either serum or urine emphasizes the necessity to investigate both fluids.
Product Categories/Family for Rabbit anti Human Ig kappa light chain (free) secondary antibody
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Product Notes
The Rabbit anti Human Ig kappa light chain (free) (Catalog #AAA571783) is a Secondary Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Human Ig kappa light chain (free) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Rabbit anti Human Ig kappa light chain (free) can be used in a range of immunoassay formats including, but not limited to, Precipitation assays. Researchers should empirically determine the suitability of the Rabbit anti Human Ig kappa light chain (free) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rabbit anti Human Ig kappa light chain (free), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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