Goat RAB8A / RAB8 Polyclonal Antibody | anti-RAB8A / RAB8 antibody

Anti-RAB8A / RAB8 Antibody (C-Terminus) IHC-plus

Cat. Num. AAA2400459

• Gene Names: RAB8A; MEL; RAB8
• Reactivity: Reactivity: Mouse, Rat, Human; Predicted Reactivity: Monkey, Canine (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Immunoaffinity purified
• Synonyms: RAB8A / RAB8; Polyclonal Antibody; Anti-RAB8A / RAB8 Antibody (C-Terminus) IHC-plus; Goat Polyclonal (IgG) to Mouse RAB8A / RAB8; Mouse RAB8A / RAB8; RAB8A; RAB8; Ras-related protein rab-8; Oncogene c-mel; Ras-associated protein RAB8; Ras-related protein Rab-8A; MEL; anti-RAB8A / RAB8 antibody

• Host: Goat
• Reactivity: Reactivity: Mouse, Rat, Human; Predicted Reactivity: Monkey, Canine (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects a band of 24 kDa by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab8a and GFP-Rab8b cds.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, 20% glycerol, 0.05% sodium azide.
• Concentration: 3 mg/ml (varies by lot)
• Sequence Length: 207

• Applicable Applications for anti-RAB8A / RAB8 antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF (1:100-1:250); IHC-P (5 ug/ml); WB (1:250-1:2000); Optimal dilution to be determined by the researcher.
• Immunogen: RAB8A/RAB8 antibody was raised against purified recombinant peptide derived from within residues 170 aa to the C-terminus of mouse Rab8a and Rab8b produced in E Coli.
• Immunogen species: RAB8A/RAB8 antibody was raised against Mouse
• Antigen Type: Recombinant protein
• Antigen Modification: C-Terminus
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-RAB8A / RAB8 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence - anti-Rab8 antibody at 1:100 dilution using primary macrophages. Cells were fixed with 4% of PFA.)

Western Blot (WB)

(Western blot. Anti-Rab8 antibody at 1:500 dilution. Lysates at 50 ug per lane. Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.)

Related Product Information for anti-RAB8A / RAB8 antibody

RAB8A Antibody, RAB8 Antibody, Ras-related protein rab-8 Antibody, Oncogene c-mel Antibody, Ras-associated protein RAB8 Antibody, Ras-related protein Rab-8A Antibody, MEL Antibody Description: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release.

NCBI and Uniprot Product Information

• NCBI GI #: 16933567
• NCBI GeneID: 4218
• NCBI Accession #: NP_005361.2
• NCBI GenBank Nucleotide #: NM_005370.4
• UniProt Accession #: P61006; P24407; Q6FHV5; B4DEK7
• Molecular Weight: 23,564 Da
• NCBI Official Full Name: ras-related protein Rab-8A
• NCBI Official Synonym Full Names: RAB8A, member RAS oncogene family
• NCBI Official Symbol: RAB8A
• NCBI Official Synonym Symbols: MEL; RAB8
• NCBI Protein Information: ras-related protein Rab-8A
• UniProt Protein Name: Ras-related protein Rab-8A
• UniProt Gene Name: RAB8A
• UniProt Synonym Gene Names: MEL; RAB8
• UniProt Entry Name: RAB8A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq, Jul 2008]

Uniprot Description

RAB8A: May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO/C1orf88. Interacts with BIRC6/bruce. Interacts with OCRL. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; Motility/polarity/chemotaxis; Oncoprotein; G protein, monomeric, Rab; G protein

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: cell soma; centriole; centrosome; cilium; cytoplasmic vesicle membrane; cytosol; dendrite; Golgi membrane; nonmotile primary cilium; nucleolus; nucleus; phagocytic vesicle; phagocytic vesicle membrane; plasma membrane; postsynaptic density; recycling endosome membrane; synaptic vesicle; trans-Golgi network transport vesicle

Molecular Function: GDP binding; GTP binding; GTPase activity; myosin V binding; protein binding; protein kinase binding; Rab GTPase binding

Biological Process: axonogenesis; cellular response to insulin stimulus; cilium biogenesis; G2/M transition of mitotic cell cycle; Golgi vesicle fusion to target membrane; mitotic cell cycle; organelle organization and biogenesis; protein secretion; regulation of long-term neuronal synaptic plasticity; regulation of protein transport; small GTPase mediated signal transduction; vesicle docking during exocytosis