Western Blot (WB)
(Western blot analysis of extracts from Mouse kidney, using RAB7L1 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit RAB7L1 Polyclonal Antibody | anti-RAB7L1 antibody
RAB7L1 Antibody
Gene Names
RAB7L1; RAB7L
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
RAB7L1; Polyclonal Antibody; RAB7L1 Antibody; DKFZp686P1051; Rab 7 like protein 1; RAB 7L; Rab-7-like protein 1; RAB29; Rab7 like protein 1; RAB7 member RAS oncogene family like 1; RAB7L; RAB7L_HUMAN; Ras related protein Rab 7L1; Ras related protein Rab7L1; Ras-related protein Rab-29; Ras-related protein Rab-7L1; anti-RAB7L1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
RAB7L1 Antibody detects endogenous levels of total RAB7L1.
Tissue Specificity: Ubiquitous.
Tissue Specificity: Ubiquitous.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-RAB7L1 antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human RAB7L1.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
In case of Salmonella enterica serovar Typhimurium (S.Typhimurium) infection, is proteolytically cleaved between Gly-41 and Val-42 by the GtgE viral protease encoded on the Gifsy-2 lysogen bacteriophage, which therefore prevents the recruitment of RAB29 to S.Typhimurium-containing vacuoles. In contrast, no proteolytically cleavage is detected in S.Typhi-infected cells.
Subunit Structure
Interacts with LRRK2.
Similarity
Belongs to the small GTPase superfamily. Rab family.
Subcellular Location
Cell membrane>Lipid-anchor>Cytoplasmic side. Cytoplasm. Cytoplasm>Perinuclear region. Golgi apparatus. Golgi apparatus>trans-Golgi network. Vacuole. Cytoplasm>Cytoskeleton.
Note: Colocalizes with LRRK2 along tubular structures emerging from Golgi apparatus (PubMed:29212815). Colocalizes with GM130 at the Golgi apparatus (PubMed:22042847). Colocalizes with dynamic tubules emerging from and retracting to the Golgi apparatus (PubMed:22042847). Colocalizes with TGN46 at the trans-Golgi network (TGN) (PubMed:24788816). In Salmonella enterica serovar Typhi (S.Typhi) infected epithelial cells, is recruited and colocalized with both S.Typhi-containing vacuoles and dynamic tubules as well as those emerging from the vacuole toward the cell periphery (PubMed:22042847).
Note: Colocalizes with LRRK2 along tubular structures emerging from Golgi apparatus (PubMed:29212815). Colocalizes with GM130 at the Golgi apparatus (PubMed:22042847). Colocalizes with dynamic tubules emerging from and retracting to the Golgi apparatus (PubMed:22042847). Colocalizes with TGN46 at the trans-Golgi network (TGN) (PubMed:24788816). In Salmonella enterica serovar Typhi (S.Typhi) infected epithelial cells, is recruited and colocalized with both S.Typhi-containing vacuoles and dynamic tubules as well as those emerging from the vacuole toward the cell periphery (PubMed:22042847).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Mouse kidney, using RAB7L1 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Mouse kidney, using RAB7L1 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-RAB7L1 antibody
The small GTPases Rab are key regulators in vesicle trafficking. Essential for maintaining the integrity of the endosome-trans-Golgi network structure (By similarity). Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Recruits LRRK2 to the Golgi complex and stimulates LRRK2 kinase activity. Regulates neuronal process morphology in the intact central nervous system (CNS) (By similarity). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,155 Da
NCBI Official Full Name
ras-related protein Rab-7L1 isoform 1
NCBI Official Synonym Full Names
RAB7, member RAS oncogene family-like 1
NCBI Official Symbol
RAB7L1
NCBI Official Synonym Symbols
RAB7L
NCBI Protein Information
ras-related protein Rab-7L1; rab-7-like protein 1
UniProt Protein Name
Ras-related protein Rab-7L1
UniProt Gene Name
RAB7L1
UniProt Entry Name
RAB7L_HUMAN
Uniprot Description
RAB7L1: Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: G protein, monomeric; G protein, monomeric, Rab; G protein
Chromosomal Location of Human Ortholog: 1q32
Cellular Component: cytoskeleton; mitochondrion; perinuclear region of cytoplasm; cytoplasm; melanosome; plasma membrane; vacuole; trans-Golgi network
Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding
Biological Process: protein transport; ER to Golgi vesicle-mediated transport; melanosome organization and biogenesis; metabolic process; cell differentiation; Rab protein signal transduction; Golgi organization and biogenesis
Research Articles on RAB7L1
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Product Notes
The RAB7L1 rab7l1 (Catalog #AAA9613798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB7L1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAB7L1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the RAB7L1 rab7l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB7L1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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