Rabbit anti-Human RAB28 Polyclonal Antibody | anti-RAB28 antibody

RAB28, ID (RAB28, Ras-related protein Rab-28) (PE)

Cat. Num. AAA6339700

• Gene Names: RAB28; CORD18
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RAB28; Polyclonal Antibody; ID (RAB28; Ras-related protein Rab-28) (PE); Ras-related protein Rab-28; anti-RAB28 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 220

• Applicable Applications for anti-RAB28 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RAB28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-147 amino acids from the Central region of human RAB28.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RAB28 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the RAB28 antibody detected the RAB28 protein (arrow).)

Related Product Information for anti-RAB28 antibody

This gene encodes a member of the Rab subfamily of Ras-related small GTPases. The encoded protein may be involved in regulating intracellular trafficking. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 9 and X.

Product Categories/Family for anti-RAB28 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Lee, S.H., et al. FEBS Lett. 582(29):4107-4111(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007). Stenmark, H., et al. Genome Biol. 2 (5), REVIEWS3007 (2001): Pereira-Leal, J.B., et al. J. Mol. Biol. 301(4):1077-1087(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 227497996
• NCBI GeneID: 9364
• NCBI Accession #: NP_004240.2; NP_001017979.1
• NCBI GenBank Nucleotide #: NP_004240.2
• UniProt Accession #: P51157
• NCBI Official Full Name: ras-related protein Rab-28 isoform 2
• NCBI Official Synonym Full Names: RAB28, member RAS oncogene family
• NCBI Official Symbol: RAB28
• NCBI Official Synonym Symbols: CORD18
• NCBI Protein Information: ras-related protein Rab-28
• UniProt Protein Name: Ras-related protein Rab-28
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB28
• UniProt Entry Name: RAB28_HUMAN

NCBI Description

This gene encodes a member of the Rab subfamily of Ras-related small GTPases. The encoded protein may be involved in regulating intracellular trafficking. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 9 and X. [provided by RefSeq, Apr 2009]

Uniprot Description

Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side

Potential. Cytoplasm › cytoskeleton › cilium basal body. Note: Expressed in the basal body and ciliary rootlet of the photoreceptors

By similarity.

Tissue specificity: Isoform S is detected in most tissues investigated: cortex, liver, kidney, skeletal muscle, adipose tissue, testis, urothelium, lung, bone marrow and retinal pigment epithelium (RPE). Isoform L 2 is widely and abundantly expressed all tissues. Isoform 3 is highly expressed in heart, lung, bone marrow, retina, brain, and RPE.

Involvement in disease: Cone-rod dystrophy 18 (CORD18) [MIM:615374]: A form of cone-rod dystrophy, an inherited retinal dystrophy characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.8

Sequence similarities: Belongs to the small GTPase superfamily. Rab family.