herpesvirus 1 ICP47 protein (US12) Recombinant Protein | US12 recombinant protein

Recombinant Human herpesvirus 1 ICP47 protein (US12)

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: herpesvirus 1 ICP47 protein (US12); Recombinant Human herpesvirus 1 ICP47 protein (US12); Immediate-early protein IE12; Immediate-early-5; Infected cell protein 47; US12 protein; Vmw12; US12 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-88aa; Full Length
• Sequence: MSWALEMADTFLDTMRVGPRTYADVRDEINKRGREDREAARTAVHDPERPLLRSPGLLPEIAPNASLGVAHRRTGGTVTDSPRNPVTR

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for US12 recombinant protein

Plays a role in the inhibition of host immune response. Binds specifically to transporters associated with antigen processing (TAP), thereby blocking peptide-binding and translocation by TAP as well as subsequent loading of peptides onto MHC class I molecules. pty MHC I molecules are retained in the endoplasmic reticulum and ultimately directed to proteasomal degradation. In consequence, infected cells are masked for immune recognition by cytotoxic T-lymphocytes.

References

DNA sequence of an immediate-early gene (IEmRNA-5) of herpes simplex virus type I.Watson R.J., Vande Woude G.F.Nucleic Acids Res. 10:979-991(1982) DNA sequence analysis of an immediate-early gene region of the herpes simplex virus type 1 genome (map coordinates 0.950 to 0.978) .Murchie M.-J., McGeoch D.J.J. Gen. Virol. 62:1-15(1982) A 3' co-terminal family of mRNAs from the herpes simplex virus type 1 short region two overlapping reading frames encode unrelated polypeptide one of which has highly reiterated amino acid sequence.Rixon F.J., McGeoch D.J.Nucleic Acids Res. 12:2473-2487(1984) Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1.McGeoch D.J., Dolan A., Donald S., Rixon F.J.J. Mol. Biol. 181:1-13(1985) The DNA sequences of the long repeat region and adjoining parts of the long unique region in the genome of herpes simplex virus type 1.Perry L.J., McGeoch D.J.J. Gen. Virol. 69:2831-2846(1988) Reiterated sequences within the intron of an immediate-early gene of herpes simplex virus type 1.Watson R.J., Umene K., Enquist L.W.Nucleic Acids Res. 9:4189-4199(1981) Herpes simplex virus type 1 (HSV-1) -induced retinitis following herpes simplex encephalitis indications for brain-to-eye transmission of HSV-1.Maertzdorf J., Van der Lelij A., Baarsma G.S., Osterhaus A.D.M.E., Verjans G.M.G.M.3.3.CO;2-Z>Ann. Neurol. 48:936-939(2000) Herpes simplex virus type 1 transmission through corneal transplantation.Remeijer L., Maertzdorf J., Doornenbal P., Verjans G.M.G.M., Osterhaus A.D.M.E.Lancet 357:442-442(2001) A cytosolic herpes simplex virus protein inhibits antigen presentation to CD8+ T lymphocytes.York I.A., Roop C., Andrews D.W., Riddell S.R., Graham F.L., Johnson D.C.Cell 77:525-535(1994) A viral inhibitor of peptide transporters for antigen presentation.Frueh K., Ahn K., Djaballah H., Sempe P., van Endert P.M., Tampe R., Peterson P.A., Yang Y.Nature 375:415-418(1995) Molecular mechanism and species specificity of TAP inhibition by herpes simplex virus ICP47.Ahn K., Meyer T.H., Uebel S., Sempe P., Djaballah H., Yang Y., Peterson P.A., Frueh K., Tampe R.EMBO J. 15:3247-3255(1996) The active domain of the herpes simplex virus protein ICP47 a potent inhibitor of the transporter associated with antigen processing (TAP) .Neumann L., Kraas W., Uebel S., Jung G., Tampe R.J. Mol. Biol. 272:484-492(1997) Inhibition of antigen transport by expression of infected cell peptide 47 (ICP47) prevents cell surface expression of HLA in choriocarcinoma cell lines.Easterfield A.J., Austen B.M., Westwood O.M.R.J. Reprod. Immunol. 50:19-40(2001) Structure of the active domain of the herpes simplex virus protein ICP47 in water/sodium dodecyl sulfate solution determined by nuclear magnetic resonance spectroscopy.Pfaender R., Neumann L., Zweckstetter M., Seger C., Holak T.A., Tampe R.Biochemistry 38:13692-13698(1999)

NCBI and Uniprot Product Information

• NCBI GI #: 820945303
• NCBI GeneID: 2703441
• NCBI Accession #: YP_009137148.1
• NCBI GenBank Nucleotide #: NC_001806.2
• UniProt Accession #: P03170; Q99BW2; Q9E0N0; Q9E0N1
• Molecular Weight: 11.8 kDa
• NCBI Official Full Name: TAP transporter inhibitor ICP47
• NCBI Official Symbol: US12
• NCBI Protein Information: inhibits antigen presentation; involved in immune regulation
• UniProt Protein Name: ICP47 protein
• UniProt Gene Name: US12
• UniProt Entry Name: ICP47_HHV11