Western Blot (WB)
(Figure 1. Western blot analysis of RAB1B using anti-RAB1B antibody (MBS1753666).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human HEK293 whole cell lysatesLane 3: human SKOV3 whole cell lysatesLane 4: human MCF-7 whole cell lysatesLane 5: human A375 whole cell lysatesLane 6: human U87 whole cell lysatesLane 7: human A549 whole cell lysaetsLane 8: rat kidney tissue lysatesLane 9: rat brain tissue lysatesLane 10: rat heart tissue lysatesLane 11: rat NRK whole cell lysatesLane 12: mouse brain tissue lysatesLane 13: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-RAB1B antigen affinity purified polyclonal antibody (Catalog # MBS1753666) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for RAB1B at approximately 24KD. The expected band size for RAB1B is at 24KD. )
Rabbit RAB1B Polyclonal Antibody | anti-RAB1B antibody
Anti-RAB1B Antibody
IHC-P: 2-5ug/ml|Human|
ICC/IF: 5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human, Mouse|
Western Blot (WB)
(Figure 1. Western blot analysis of RAB1B using anti-RAB1B antibody (MBS1753666).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human HEK293 whole cell lysatesLane 3: human SKOV3 whole cell lysatesLane 4: human MCF-7 whole cell lysatesLane 5: human A375 whole cell lysatesLane 6: human U87 whole cell lysatesLane 7: human A549 whole cell lysaetsLane 8: rat kidney tissue lysatesLane 9: rat brain tissue lysatesLane 10: rat heart tissue lysatesLane 11: rat NRK whole cell lysatesLane 12: mouse brain tissue lysatesLane 13: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-RAB1B antigen affinity purified polyclonal antibody (Catalog # MBS1753666) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for RAB1B at approximately 24KD. The expected band size for RAB1B is at 24KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of RAB1B using anti-RAB1B antibody (MBS1753666).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human HEK293 whole cell lysatesLane 3: human SKOV3 whole cell lysatesLane 4: human MCF-7 whole cell lysatesLane 5: human A375 whole cell lysatesLane 6: human U87 whole cell lysatesLane 7: human A549 whole cell lysaetsLane 8: rat kidney tissue lysatesLane 9: rat brain tissue lysatesLane 10: rat heart tissue lysatesLane 11: rat NRK whole cell lysatesLane 12: mouse brain tissue lysatesLane 13: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-RAB1B antigen affinity purified polyclonal antibody (Catalog # MBS1753666) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for RAB1B at approximately 24KD. The expected band size for RAB1B is at 24KD. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of RAB1B using anti-RAB1B antibody (MBS1753666).RAB1B was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-RAB1B Antibody (MBS1753666) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of RAB1B using anti-RAB1B antibody (MBS1753666).RAB1B was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-RAB1B Antibody (MBS1753666) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of RAB1B using anti-RAB1B antibody (MBS1753666).RAB1B was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-RAB1B Antibody (MBS1753666) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of RAB1B using anti-RAB1B antibody (MBS1753666).RAB1B was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-RAB1B Antibody (MBS1753666) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of RAB1B using anti-RAB1B antibody (MBS1753666).RAB1B was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-RAB1B Antibody (MBS1753666) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of RAB1B using anti-RAB1B antibody (MBS1753666).RAB1B was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-RAB1B Antibody (MBS1753666) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 5. IHC analysis of RAB1B using anti-RAB1B antibody (MBS1753666).RAB1B was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-RAB1B Antibody (MBS1753666) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 5. IHC analysis of RAB1B using anti-RAB1B antibody (MBS1753666).RAB1B was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-RAB1B Antibody (MBS1753666) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 6. IHC analysis of RAB1B using anti-RAB1B antibody (MBS1753666).RAB1B was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-RAB1B Antibody (MBS1753666) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 6. IHC analysis of RAB1B using anti-RAB1B antibody (MBS1753666).RAB1B was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-RAB1B Antibody (MBS1753666) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunofluorescence (IF)
(Figure 7. IF analysis of RAB1B using anti- RAB1B antibody (MBS1753666).RAB1B was detected in immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-RAB1B Antibody (MBS1753666) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Immunofluorescence (IF)
(Figure 7. IF analysis of RAB1B using anti- RAB1B antibody (MBS1753666).RAB1B was detected in immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-RAB1B Antibody (MBS1753666) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Flow Cytometry (FC/FACS)
(Figure 8. Flow Cytometry analysis of HEPA1-6 cells using anti-RAB1B antibody (MBS1753666).Overlay histogram showing HEPA1-6 cells stained with MBS1753666 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB1B Antibody (MBS1753666, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 8. Flow Cytometry analysis of HEPA1-6 cells using anti-RAB1B antibody (MBS1753666).Overlay histogram showing HEPA1-6 cells stained with MBS1753666 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB1B Antibody (MBS1753666, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 9. Flow Cytometry analysis of U251 cells using anti-RAB1B antibody (MBS1753666).Overlay histogram showing U251 cells stained with MBS1753666 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB1B Antibody (MBS1753666, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 9. Flow Cytometry analysis of U251 cells using anti-RAB1B antibody (MBS1753666).Overlay histogram showing U251 cells stained with MBS1753666 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB1B Antibody (MBS1753666, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Chen, D., Guo, J., Gahl, W. A. RAB GTPases expressed in human melanoma cells. Biochim. Biophys. Acta 1355: 1-6, 1997.
3. Hartz, P. A. Personal Communication. Baltimore, Md. 1/29/2009.
NCBI and Uniprot Product Information
NCBI Description
Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).[supplied by OMIM, Jan 2009]
Uniprot Description
RAB1B: Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Belongs to the small GTPase superfamily. Rab family.
Protein type: G protein, monomeric, Rab; G protein; G protein, monomeric
Chromosomal Location of Human Ortholog: 11q12
Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; mitochondrion; pre-autophagosomal structure membrane; ER-Golgi intermediate compartment
Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding
Biological Process: intracellular protein transport; ER to Golgi vesicle-mediated transport; autophagy; virus assembly; Rab protein signal transduction
Research Articles on RAB1B
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Product Notes
The RAB1B rab1b (Catalog #AAA1753666) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RAB1B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAB1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM). WB: 0.25-0.5ug/ml|Human, Mouse, Rat| IHC-P: 2-5ug/ml|Human| ICC/IF: 5ug/ml|Human| FC/FACS/FCM: 1-3ug/1x106 cells|Human, Mouse|. Researchers should empirically determine the suitability of the RAB1B rab1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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