Rabbit RAB13 Polyclonal Antibody | anti-RAB13 antibody

RAB13 Rabbit pAb

Cat. Num. AAA8547973

• Gene Names: RAB13; GIG4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: RAB13; Polyclonal Antibody; RAB13 Rabbit pAb; GIG4; anti-RAB13 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
• Sequence Length: 122

• Applicable Applications for anti-RAB13 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000-1:2000
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-203 of human RAB13 (NP_002861.1).
• Cellular Localization: Cell Junction, Cell Membrane, Cell Projection, Cytoplasmic Vesicle, Endosome, Golgi Apparatus, Membrane, Tight Junction
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Related Product Information for anti-RAB13 antibody

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis.

NCBI and Uniprot Product Information

• NCBI GI #: 440309849
• NCBI GeneID: 5872
• NCBI Accession #: NP_001258967.1
• NCBI GenBank Nucleotide #: NP_001258967.1
• UniProt Accession #: P51153
• Molecular Weight: 23kDa
• NCBI Official Full Name: ras-related protein Rab-13 isoform 2
• NCBI Official Synonym Full Names: RAB13, member RAS oncogene family
• NCBI Official Symbol: RAB13
• NCBI Official Synonym Symbols: GIG4
• NCBI Protein Information: ras-related protein Rab-13
• UniProt Protein Name: Ras-related protein Rab-13
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB13
• UniProt Entry Name: RAB13_HUMAN

NCBI Description

This gene is a member of the Rab family of small G proteins and plays a role in regulating membrane trafficking between trans-Golgi network (TGN) and recycling endosomes (RE). The encoded protein is involved in the assembly of tight junctions, which are components of the apical junctional complex (AJC) of epithelial cells. The AJC plays a role in forming a barrier between luminal contents and the underlying tissue. Additional functions associated with the protein include endocytic recycling of occludin, regulation of epithelial cell scattering, neuronal regeneration and regulation of neurite outgrowth. Alternately spliced transcript variants have been observed for this gene. A pseudogene associated with this gene is located on chromosome 12. [provided by RefSeq, Jan 2013]

Uniprot Description

RAB13: Could participate in polarized transport, in the assembly and/or the activity of tight junctions. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: Golgi apparatus; neuron projection; cytoplasmic vesicle membrane; tight junction; recycling endosome membrane; trans-Golgi network; recycling endosome; lamellipodium; endocytic vesicle; cytoplasm; plasma membrane; cytoplasmic vesicle; lateral plasma membrane

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: intracellular protein transport; cellular response to insulin stimulus; metabolic process; cortical actin cytoskeleton organization and biogenesis; Rab protein signal transduction; endocytic recycling; endosome transport; neurite development