Rabbit anti-Human, Mouse RAB13 Polyclonal Antibody | anti-RAB13 antibody

RAB13, ID (Ras-related Protein Rab-13, Cell Growth-inhibiting Gene 4 Protein, GIG4) (AP)

Cat. Num. AAA6496375

• Gene Names: RAB13; GIG4
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: RAB13; Polyclonal Antibody; ID (Ras-related Protein Rab-13; Cell Growth-inhibiting Gene 4 Protein; GIG4) (AP); anti-RAB13 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RAB13. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-RAB13 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human RAB13 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RAB13 antibody (Center) in mouse bladder tissue lysates (35ug/lane). RAB13 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with RAB13 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells using RAB13 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-RAB13 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

1. Nokes, R.L., J. Cell Biol. 182(5), 845-853 (2008). 2. Kanda, I., Oncogene 27(12), 1687-1695 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4506363
• NCBI GeneID: 5872
• NCBI Accession #: NP_002861
• NCBI GenBank Nucleotide #: NM_002870.3
• UniProt Accession #: P51153; Q5U0A6; Q6GPG6; Q96GU4; A8K6B5; D3DV67
• Molecular Weight: 24.7kDa (220aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ras-related protein Rab-13 isoform 1
• NCBI Official Synonym Full Names: RAB13, member RAS oncogene family
• NCBI Official Symbol: RAB13
• NCBI Official Synonym Symbols: GIG4
• NCBI Protein Information: ras-related protein Rab-13
• UniProt Protein Name: Ras-related protein Rab-13
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB13
• UniProt Entry Name: RAB13_HUMAN

NCBI Description

This gene is a member of the Rab family of small G proteins and plays a role in regulating membrane trafficking between trans-Golgi network (TGN) and recycling endosomes (RE). The encoded protein is involved in the assembly of tight junctions, which are components of the apical junctional complex (AJC) of epithelial cells. The AJC plays a role in forming a barrier between luminal contents and the underlying tissue. Additional functions associated with the protein include endocytic recycling of occludin, regulation of epithelial cell scattering, neuronal regeneration and regulation of neurite outgrowth. Alternately spliced transcript variants have been observed for this gene. A pseudogene associated with this gene is located on chromosome 12. [provided by RefSeq, Jan 2013]

Uniprot Description

RAB13: Could participate in polarized transport, in the assembly and/or the activity of tight junctions. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: Golgi apparatus; neuron projection; cytoplasmic vesicle membrane; tight junction; recycling endosome membrane; trans-Golgi network; recycling endosome; lamellipodium; endocytic vesicle; cytoplasm; plasma membrane; cytoplasmic vesicle; lateral plasma membrane

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: intracellular protein transport; cellular response to insulin stimulus; metabolic process; cortical actin cytoskeleton organization and biogenesis; Rab protein signal transduction; endocytic recycling; endosome transport; neurite development