Rabbit anti-Human QTRTD1 Polyclonal Antibody | anti-QTRTD1 antibody

QTRTD1, CT (QTRTD1, Queuine tRNA-ribosyltransferase subunit QTRTD1, Queuine tRNA-ribosyltransferase domain-containing protein 1) (PE)

Cat. Num. AAA6339491

• Gene Names: QTRT2; QTRTD1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: QTRTD1; Polyclonal Antibody; CT (QTRTD1; Queuine tRNA-ribosyltransferase subunit QTRTD1; Queuine tRNA-ribosyltransferase domain-containing protein 1) (PE); Queuine tRNA-ribosyltransferase domain-containing protein 1; anti-QTRTD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 415

• Applicable Applications for anti-QTRTD1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IF: 1:10-50; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: QTRTD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 328-359 amino acids from the C-terminal region of human QTRTD1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(QTRTD1 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the QTRTD1 antibody detected the QTRTD1 protein (arrow).)

Immunohistochemistry (IHC)

(QTRTD1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of QTRTD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of QTRTD1 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(QTRTD1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-QTRTD1 antibody

Interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp,-Asn,-His and-Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (By similarity).

Product Categories/Family for anti-QTRTD1 antibody

Antibodies; Enzymes; Ribosyltransferase

References

Chen, Y.C., et al. RNA 16(5):958-968(2010). Lamesch, P., et al. Genomics 89(3):307-315(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 13375872
• NCBI GeneID: 79691
• NCBI Accession #: NP_078914.1
• NCBI GenBank Nucleotide #: NP_078914.1
• UniProt Accession #: Q9H974
• NCBI Official Full Name: queuine tRNA-ribosyltransferase accessory subunit 2 isoform 1
• NCBI Official Synonym Full Names: queuine tRNA-ribosyltransferase accessory subunit 2
• NCBI Official Symbol: QTRT2
• NCBI Official Synonym Symbols: QTRTD1
• NCBI Protein Information: queuine tRNA-ribosyltransferase accessory subunit 2
• UniProt Protein Name: Queuine tRNA-ribosyltransferase subunit QTRTD1
• UniProt Gene Name: QTRTD1
• UniProt Entry Name: QTRD1_HUMAN

NCBI Description

This gene encodes a subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine, and tyrosine. The encoded protein may play a role in the queuosine 5'-monophosphate salvage pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

QTRTD1: Interacts with QTRT1 to form an active queuine tRNA- ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1- yl)amino)methyl)-7-deazaguanosine). Belongs to the queuine tRNA-ribosyltransferase family. QTRTD1 subfamily.

Protein type: EC 2.4.2.29

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: cytoplasm; mitochondrial outer membrane; mitochondrion

Molecular Function: protein heterodimerization activity; protein homodimerization activity; queuine tRNA-ribosyltransferase activity

Biological Process: queuosine biosynthetic process; tRNA modification