Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ANKRD28 antibody (MBS9128557) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Rabbit anti-Human, Mouse ANKRD28 Polyclonal Antibody | anti-ANKRD28 antibody
ANKRD28 Polyclonal Antibody
Gene Names
ANKRD28; PITK; PPP1R65
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purification
Synonyms
ANKRD28; Polyclonal Antibody; ANKRD28 Polyclonal Antibody; anti-ANKRD28 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence Length
1,083
Applicable Applications for anti-ANKRD28 antibody
Western Blot (WB)
Application Notes
WB 1:500 - 1:2000
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ANKRD28 (NP_056014.2).
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ANKRD28 antibody (MBS9128557) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ANKRD28 antibody (MBS9128557) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 96kDa/112kDa/116kDa
Observed MW: 113kDa
Observed MW: 113kDa
NCBI Official Full Name
serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A isoform b
NCBI Official Synonym Full Names
ankyrin repeat domain 28
NCBI Official Symbol
ANKRD28
NCBI Official Synonym Symbols
PITK; PPP1R65
NCBI Protein Information
serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A
UniProt Protein Name
Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A
Protein Family
UniProt Gene Name
ANKRD28
UniProt Synonym Gene Names
KIAA0379; PP6-ARS-A; Serine/threonine-protein phosphatase 6 regulatory subunit ARS-A; PITK
UniProt Entry Name
ANR28_HUMAN
Uniprot Description
ANKRD28: Putative regulatory subunit of protein phosphatase 6 (PP6) that may be involved in the recognition of phosphoprotein substrates. Involved in the PP6-mediated dephosphorylation of NFKBIE opposing its degradation in response to TNF-alpha. Selectively inhibits the phosphatase activity of PPP1C. Targets PPP1C to modulate HNRPK phosphorylation. 4 isoforms of the human protein are produced by alternative promoter.
Protein type: Protein phosphatase, regulatory subunit
Chromosomal Location of Human Ortholog: 3p25.1
Cellular Component: nucleoplasm
Molecular Function: protein binding
Research Articles on ANKRD28
Similar Products
Product Notes
The ANKRD28 ankrd28 (Catalog #AAA9128557) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ANKRD28 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ANKRD28 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB 1:500 - 1:2000. Researchers should empirically determine the suitability of the ANKRD28 ankrd28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANKRD28, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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