Rabbit anti-Human Pyruvate Dehydrogenase Phosphatase (PDP) Polyclonal Antibody | anti-PDP antibody

Polyclonal Antibody to Pyruvate Dehydrogenase Phosphatase (PDP)

Cat. Num. AAA2015383

• Gene Names: PDP1; PDH; PDP; PDPC; PPM2A; PPM2C
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Pyruvate Dehydrogenase Phosphatase (PDP); Polyclonal Antibody; Polyclonal Antibody to Pyruvate Dehydrogenase Phosphatase (PDP); anti-PDP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against PDP. It has been selected for its ability to recognize PDP in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NAQNER ELERLKLEHP KSEAKSVVKQ DRLLGLLMPF RAFGDVKFKW SIDLQKRVIE SGPDQLNDNE YTKFIPPNYH TPPYLTAEPE VTYHRLRPQD KFLVLATDGL WETMHRQDVV RIVGEYLTGM HHQQPIAVGG YKVTLGQMHG LLTERRTKMS SVFEDQNAAT HLIRHAVGNN EFGTVDHERL SKMLSLPEEL
• Sequence Length: 475

• Applicable Applications for anti-PDP antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant PDP (Asn315~Glu537) expressed in E.coli.
• Cross Reactivity: Human
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2049176
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 239985422
• NCBI GeneID: 54704
• NCBI Accession #: NP_001155251.1
• NCBI GenBank Nucleotide #: NM_001161779.1
• UniProt Accession #: Q9P0J1; Q5U5K1; B3KX71; J3KPU0
• Molecular Weight: 63,695 Da
• NCBI Official Full Name: pyruvate dehyrogenase phosphatase catalytic subunit 1 isoform 2
• NCBI Official Synonym Full Names: pyruvate dehyrogenase phosphatase catalytic subunit 1
• NCBI Official Symbol: PDP1
• NCBI Official Synonym Symbols: PDH; PDP; PDPC; PPM2A; PPM2C
• NCBI Protein Information: pyruvate dehyrogenase phosphatase catalytic subunit 1
• UniProt Protein Name: [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
• Protein Family: Petal death protein
• UniProt Gene Name: PDP1
• UniProt Synonym Gene Names: PDP; PPM2C; PDP 1; PDPC 1

NCBI Description

Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]

Uniprot Description

PDP1: a protein phosphoserine phosphatase associated with the mitochondrial matrix that activates phosphorylated pyruvate dehydrogenase complex by dephosphorylation. The PDPs play crucial roles in switching metabolic flux from glycolysis towards oxidative phosphorylation. Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex . PDP1 is composed of a catalytic subunit and a regulatory subunit (PDPR). Heterodimer of a catalytic subunit and a FAD protein of unknown function . Defects in PDP1 are the cause of pyruvate dehydrogenase phosphatase deficiency (PDP deficiency). PDP deficiency results in lactic acidosis leading to neurological dysfunction. Belongs to the PP2C family.

Protein type: EC 3.1.3.43; Mitochondrial; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: mitochondrial matrix

Molecular Function: [pyruvate dehydrogenase (lipoamide)] phosphatase activity; metal ion binding; protein binding; protein serine/threonine phosphatase activity

Biological Process: regulation of acetyl-CoA biosynthetic process from pyruvate

Disease: Pyruvate Dehydrogenase Phosphatase Deficiency