Flow Cytometry (FC/FACS)
(Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD15 (MEM-158) PerCP.)
Mouse anti-Human CD15 Antibody | anti-CD15 antibody
Anti-Hu CD15 PerCP
Gene Names
FUT4; LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with PerCP under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD15; Anti-Hu CD15 PerCP; fucosyltransferase 4; Lewis x Blood Group antigen; Le(x); SSEA-1; 3-fucosyl-N-acetyllactosamine; anti-CD15 antibody
Host
Mouse
Reactivity
Human
Isotype
IgM
Clone Number
MEM-158
Specificity
The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on the surface of granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells.
Purity/Purification
The purified antibody is conjugated with PerCP under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing Tris buffered saline (TBS) solution with 15 mM sodium azide
Sequence Length
530
Applicable Applications for anti-CD15 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: QC tested
Immunogen
Human granulocytes
Negative Species
Pig, Sheep, Cow
Conjugation
PerCP
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
Flow Cytometry (FC/FACS)
(Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD15 (MEM-158) PerCP.)
Flow Cytometry (FC/FACS)
(Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD15 (MEM-158) PerCP.)
Related Product Information for anti-CD15 antibody
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm identification of Hodgkin's disease.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
alpha-(1,3)-fucosyltransferase 4
NCBI Official Synonym Full Names
fucosyltransferase 4
NCBI Official Symbol
FUT4
NCBI Official Synonym Symbols
LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
NCBI Protein Information
alpha-(1,3)-fucosyltransferase 4
UniProt Protein Name
Alpha-(1,3)-fucosyltransferase 4
Protein Family
UniProt Gene Name
FUT4
UniProt Synonym Gene Names
ELFT; FCT3A; Fuc-TIV; FucT-IV
NCBI Description
The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]
Uniprot Description
May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
Research Articles on CD15
Similar Products
Product Notes
The CD15 fut4 (Catalog #AAA1801061) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD15 PerCP reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD15 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: QC tested. Researchers should empirically determine the suitability of the CD15 fut4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD15, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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